Human HDL ELISA Kit
Detection method: Colorimetric
Cell culture supernatant, Milk, Serum, Plasma
Test type: Competitive
Sensitivity: 6 µg / ml
6.25 µg / ml – 400 µg / ml
Test time: 3 h 00 min
Trial duration: Standard multi-step test
Reacts with: Human
Abcam’s competitive in vitro ELISA (enzyme-linked immunosorbent assay) kit for human high-density lipoproteins (HDL) is designed for the quantitative measurement of human HDL in plasma, serum, milk, and cell culture supernatant. An HDL-specific antibody has been precoated on 96-well plates and blocked. Standards or test samples are added to the wells and then biotinylated HDL is added and then washed with wash buffer.
The streptavidin-peroxidase complex is added and the unbound conjugates are washed with wash buffer. TMB is then used to visualize the streptavidin-peroxidase enzyme reaction. TMB is catalyzed by streptavidin-peroxidase to produce a blue product that changes to yellow after adding an acid stop solution. The density of the yellow coloration is inversely proportional to the amount of HDL captured on the plate. The complete kit can be stored at -20 ° C for long-term storage before reconstitution. Avoid repeated freeze-thaw cycles.
Store at -20 ° C. See protocols.
High-density lipoprotein (HDL) is one of the five main groups of lipoproteins, which, in order of size, from largest to smallest, are chylomicrons, VLDL, IDL, LDL, and HDL, which allow the transport of lipids such as cholesterol and triglycerides. water-based into the bloodstream. In healthy individuals, approximately thirty percent of the cholesterol in the blood is transported by HDL.
Blood tests usually report the level of HDL-C, that is, the amount of cholesterol that the HDL particles contain. It is often contrasted with LDL or low-density cholesterol or LDL-C. HDL particles are capable of removing cholesterol from the interior of the arterial atheroma and transporting it back to the liver for excretion or reuse, which is the main reason why cholesterol transported within HDL particles (HDL-C) to It is sometimes called “good cholesterol” (even though it is exactly the same as the cholesterol in LDL particles).