Serine protease hepsin (PNH), ELISA kit
Full Product Name
Mouse Serine Protease Hepsin (PNH) ELISA Kit
Product synonym names
TMPRSS1; hepsin serine protease catalytic chain; non-catalytic chain of serine protease hepsin; transmembrane protease; serine 1
Product gene name
Elisa HPN Kit
ModBase 3D Structure for O35453
Species reactivity: Mouse
This assay has high sensitivity and excellent specificity for the detection of mouse PNH. No significant cross-reactivity or interference was observed between mouse PNH and analogs.
Test type: Quantitative sandwich
Serum, plasma, other biological fluids
Detection wavelength: 450 nm
Intra-assay precision (precision within an assay)
Three samples of known concentration were run twenty times on a plate to assess intra-assay precision.
Inter-assay precision (precision between assays)
Three samples of known concentration were analyzed in forty separate tests to assess precision between tests.
CV (%): = SD / meanX100
Preparation and storage
Store at 2-8 degrees C.
Information selected from the online data sheet is extracted from bioinformatics databases, occasionally resulting in the ambiguous or irrelevant product information. It is the customer’s responsibility to review, verify and evaluate the information to make sure it matches their requirements before purchasing the kit. The essays in our ELISA kit are dynamic research tools and can sometimes be updated and improved.
If the format of this assay is important to you, please request the current manual or contact our technical support team with a pre-sale consultation before placing an order. We will confirm the current details of the trial. We cannot guarantee that the sample manual posted online is the most current manual, it is intended to serve as an example only. See the instructions for use provided with the test kit for precise details.
Occasionally, small volumes of HPN ELISA kit vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly centrifuge the vial in a benchtop centrifuge to dislodge any liquid on the lid of the container. Some products may need to be shipped with dry ice and an additional fee may apply for dry ice.
Search terms for purchasing HPN
MBS287067 is a ready-to-use microwell plate ELISA (enzyme-linked immunosorbent assay) kit for testing the presence of target analytes from the Serine Protease Hepsin (PNH) ELISA Kit in biological samples. The concentration gradients of the kit standards or positive controls provide a theoretical detection range of the kit in biological research samples containing PNH.
The analytical biochemical ELISA technique of the MBS287067 kit is based on HPN antibody-HPN antigen interactions (immunoadsorption) and an HRP colorimetric detection system to detect HPN antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant PNH. Appropriate sample types may include secretions and undiluted body fluids and/or tissue homogenates. Quality control trials evaluating reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
Related Product Information for Elisa PNH kit
This assay uses a two-site sandwich ELISA to quantify PNH in samples. A microplate has been pre-coated with an antibody specific for PNH. Standards and samples are pipetted into the wells and any PNH present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNH is added to the wells. After washing, streptavidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and the color develops in proportion to the amount of PNH bound in the initial step. The color development is stopped and the intensity of the color is measured.