We reveal extremely delicate and selective chemiresistive-type NO fuel detection utilizing defected single-walled carbon nanotubes (SWCNTs) embellished with N-[3-(trimethoxysilyl)propyl]ethylene diamine (en-APTAS) molecules. The defected SWCNTs have been ready through furnace annealing at 700 °C and confirmed by transmission electron microscopy. A single en-APTAS molecule has two amine teams performing as adsorption websites for NO fuel, which might enhance the NO response. The NO response was additional enhanced when the defected SWCNTs have been utilized as a result of NO sensing reactions might happen on each the inside and outer partitions of the defected SWCNTs.
The en-APTAS ornament improved the NO response of the SWCNT-based fuel sensing units by 2.5 occasions; when the defected SWCNTs have been used, the NO response was additional improved by three occasions. In the meantime, the restoration efficiency in a time-resolved response curve was considerably improved (45 occasions) through a easy rinsing course of with ethanol. Particularly, the fabricated gadget didn’t reply to carbon monoxide (CO) or BTEX fuel (i.e., a combination of benzene, toluene, ethyl benzene, and xylene), indicating its excessive selectivity to NO fuel. The outcomes present the potential of a high-performance SWCNT-based NO fuel sensor relevant to healthcare fields requiring ppb-level detection, comparable to in vitro diagnostics (IVDs) of respiratory illnesses.
Lung most cancers is likely one of the most generally unfold cancers on this planet and half of the non-small cell lung cancers are lung adenocarcinoma (LUAD). Though there have been a number of medicine been permitted for LUAD remedy, a big portion of LUAD nonetheless can’t be successfully handled as a consequence of lack of obtainable therapeutic targets. Right here, we investigated the oncogenic roles of DKC1 in LUAD and its potential mechanism and explored the potential of focusing on DKC1 for LUAD remedy. DKC1 is overexpressed in LUAD in contrast with adjoining regular tissues.
Excessive expression of DKC1 predicts the poor total survival. DKC1 knockdown in LUAD cell traces induced G1 part arrest and inhibited cell proliferation. Ectopic expression of DKC1 might rescue the expansion of LUAD cell traces. As well as, the abundance of DKC1 is positively correlated with telomerase RNA part (TERC) and telomerase reverse transcriptase (TERT) ranges in LUAD. DKC1 downregulation resulted in decreased TERC expression, decreased telomerase exercise and shorten telomere, and thus finally led to cell senescence and apoptosis.
Elevated miR-21-3p and miR-487b-3p serum ranges throughout anaphylactic response in meals allergic kids
Anaphylaxis is essentially the most extreme manifestation of allergic issues. The poor information of its molecular mechanisms usually results in underdiagnosis. MicroRNAs (miRNA) regulate physiological and pathological processes they usually have been postulated as promising diagnostic markers. The primary aims of this research are to characterize the human miRNA profile throughout anaphylaxis and assess their capability as diagnostic markers in addition to decide their participation within the molecular mechanisms of this occasion.
The miRNA serum profiles from the acute and baseline part of 5 oral meals challenged-anaphylactic kids (<18 years previous) have been obtained by Subsequent Technology Sequencing (NGS). From the panel of statistically vital miRNAs obtained, a number of candidates have been chosen and analyzed in 19 anaphylactic kids by qPCR. We carried out Techniques Biology Evaluation (SBA) on their goal genes to determine major features and canonical pathways. A purposeful in vitro assay was carried out incubating endothelial cells (ECs) in anaphylactic situations.
The NGS recognized 389 miRNAs amongst which 41 have been considerably totally different between acute and baseline samples. The excessive ranges of miR-21-3p (fold change=2.28, p=0.006) and miR-487b-3p (fold change=1.04, p=0.039) noticed by NGS in acute serum samples have been confirmed in a bigger group of 19 sufferers. The SBA revealed molecular pathways associated with the irritation and immune system regulation. miR-21-3p elevated intracellularly and in acute part serum after EC stimulation.
These findings present, for the primary time, some insights into the anaphylactic miRNA serum profile in kids and level to miR-21-3p and miR-487b-3p as candidate biomarkers. Moreover, the SBA revealed a attainable implication of those molecules within the underlying molecular mechanisms. Furthermore, ECs elevated miR-21-3p intracellularly and launched it to the surroundings in response to anaphylaxis.
Checks for tuberculosis an infection: panorama evaluation
Solely tuberculin pores and skin checks (TST) and two interferon-γ launch assays (IGRA) – QuantiFERON-TB Gold In-Tube and T-SPOT.TB – are presently endorsed by the World Well being Group as checks for tuberculosis (TB) an infection. Whereas IGRAs are extra particular than TST, they require subtle laboratory infrastructure and are pricey to carry out. Checks with improved predictive efficiency and operational traits are wanted.We reviewed the present panorama of checks for TB an infection recognized via a web-based survey focusing on diagnostic producers globally.We recognized 20 checks for TB an infection together with 15 in-vitro checks and 5 pores and skin checks.
13 of the in-vitro checks are whole-blood IGRA and 14 makes use of early secreted antigenic goal 6 (ESAT-6) and tradition filtrate protein 10 (CFP-10), with or with out extra antigens. Ten are primarily based on assays apart from an enzyme-linked immunosorbent assay similar to a fluorescent lateral circulate assay, which requires much less guide operation and shorter assay time and therefore is extra appropriate for decentralisation in comparison with the prevailing IGRA.4 of the 5 pores and skin checks use ESAT-6 and CFP-10 proteins whereas the remaining one makes use of a brand new antigen that’s particular to Mycobacterium tuberculosis complicated.
OxiSelect ORAC Activity Assay Kit |
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| STA-345-5 | Cell Biolabs | 5 x 192 assays | EUR 2277 |
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Description: The ORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect ORAC Activity Assay measures this capacity in less than 2 hours from a wide variety of sample types. |
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OxiSelect HORAC Activity Assay Kit |
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| STA-346 | Cell Biolabs | 192 assays | EUR 577 |
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Description: The HORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect HORAC Activity Assay measures the degradation of free hydroxyl radicals in less than 2 hours from a wide variety of sample types. |
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OxiSelect HORAC Activity Assay Kit |
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| STA-346-5 | Cell Biolabs | 5 x 192 assays | EUR 2277 |
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Description: The HORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect HORAC Activity Assay measures the degradation of free hydroxyl radicals in less than 2 hours from a wide variety of sample types. |
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OxiSelect Comet Assay Control Cells |
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| STA-354 | Cell Biolabs | 1 set | EUR 253 |
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Description: OxiSelect Comet Assay Control Cells are a set of two cell lines that serve as a positive control (Etoposide-treated) and a negative control (untreated) respectively. |
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OxiSelect Protein Radical ELISA Kit |
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| STA-810 | Cell Biolabs | 96 assays | EUR 589 |
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Description: Protein radicals form from electron transfer from various reactive oxygen and reactive nitrogen species. Protein radicals have been linked to various disorders including amyotrophic lateral sclerosis, Huntington's Disease and Alzheimer's disease. The OxiSelect Protein Radical ELISA Kit quantifies free radicals in a standard 96-well plate. |
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OxiSelect Glutathione Reductase Assay Kit |
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| STA-812 | Cell Biolabs | 100 assays | EUR 421 |
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Description: Cell Biolabs? OxiSelect Glutathione Reductase Assay Kit is a Glutathione reductase activity is defined as 1 unit of enzyme reducing 1 µmole oxidized glutathione (GSSG) per minute at pH 7.6 and 25ºC. The kit employs a simple enzymatic recycling reaction for glutathione quantification where the reduction of a chromagen is correlated to glutathione reductase enzymatic activity. The kit has a detection sensitivity limit of approximately 0.6 mU/mL. Each kit provides sufficient reagents to perform up to 100 assays, including standard curve and unknown samples. |
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OxiSelect Polyamine Oxidase Assay Kit |
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| XPX-5107 | Cell Biolabs | 100 assays | EUR 450 |
OxiSelect BPDE Protein Adduct ELISA Kit |
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| STA-301 | Cell Biolabs | 96 assays | EUR 786 |
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Description: BSA standards or protein samples (10 ?g/mL) are adsorbed onto a 96-well plate for 2 hrs at 37ºC. The BPDE-protein adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody. The BPDE protein adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-BSA standards. |
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OxiSelect Nitrotyrosine ELISA Kit (1 plate) |
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| STA-305 | Cell Biolabs | 96 assays | EUR 798 |
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Description: Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect Nitrotyrosine ELISA Kit provides a sensitive method to measure the formation of 3-nitrotyrosine in proteins. |
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OxiSelect Nitrotyrosine ELISA Kit (5 plates) |
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| STA-305-5 | Cell Biolabs | 5 x 96 assays | EUR 3240 |
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Description: Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect Nitrotyrosine ELISA Kit provides a sensitive method to measure the formation of 3-nitrotyrosine in proteins. |
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OxiSelect Nitrotyrosine ELISA Kit, Trial Size |
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| STA-305-T | Cell Biolabs | 32 assays | EUR 421 |
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Description: Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect Nitrotyrosine ELISA Kit provides a sensitive method to measure the formation of 3-nitrotyrosine in proteins. |
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OxiSelect AOPP Assay Kit (200 assays) |
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| STA-318 | Cell Biolabs | 200 assays | EUR 635 |
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Description: Advanced Oxidation Protein Products (AOPP) are uremic toxins created during oxidative stress through the reaction of plasma proteins with chlorinated oxidants such as chloramines or hypochlorous acid. AOPP levels are elevated in patients with renal complications, diabetes mellitus, and atherosclerosis, as well as HIV-positive patients. Our OxiSelect AOPP Assay Kit provides a convenient method to assess oxidative stress. The kit includes a Chloramine standard and an AOPP Human Serum Albumin conjugate for use as a positive control. |
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OxiSelect TBARS Assay Kit (MDA Quantitation) |
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| STA-330 | Cell Biolabs | 200 assays | EUR 659 |
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Description: The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE. |
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OxiSelect TBARS Assay Kit (MDA Quantitation) |
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| STA-330-5 | Cell Biolabs | 5 x 200 assays | EUR 2671 |
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Description: The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE. |
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OxiSelect Catalase Activity Assay Kit, Fluorometric |
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| STA-339 | Cell Biolabs | 500 assays | EUR 757 |
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Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kit measures catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 500 assays in a 96-well microtiter plate. This includes blanks, catalase standards and unknown samples. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay. |
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OxiSelect Superoxide Dismutase Activity Assay Kit |
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| STA-340 | Cell Biolabs | 100 assays | EUR 635 |
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Description: Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidant enzymes. SOD enzymes are classified into three groups: cytosolic CuZn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our OxiSelect Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions and a chromagen to produce a water-soluble formazan dye upon reduction by superoxide anions. The superoxide dismutase activity is determined as the inhibition or reduction of chromagen. |
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OxiSelect Catalase Activity Assay Kit (Colorimetric) |
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| STA-341 | Cell Biolabs | 96 assays | EUR 676 |
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Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kits measure catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 100 assays in a 96-well microtiter plate, or 50 assays in microcentrifuge tubes. This includes blanks, catalase standards and unknown samples. Direct spectrophotometric detection of catalase activity with ultraviolet light can cause interference from proteins and other biological components. The OxiSelect Catalase Activity Assay Kit (Colorimetric) utilizes visible light (520 nm), which reduces sample interference. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay. |
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OxiSelect Comet Assay Slides (3-Well) |
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| STA-352 | Cell Biolabs | 5 slides | EUR 218 |
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Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
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OxiSelect Comet Assay Slides (3-Well) |
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| STA-353 | Cell Biolabs | 25 slides | EUR 363 |
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Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
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OxiSelect Comet Assay Slides (3-Well) |
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| STA-353-5 | Cell Biolabs | 5 x 25 slides | EUR 1268 |
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Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
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OxiSelect 96-Well Comet Assay Kit |
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| STA-355 | Cell Biolabs | 96 assays | EUR 427 |
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Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
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OxiSelect 96-Well Comet Assay Kit |
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| STA-355-5 | Cell Biolabs | 5 x 96 assays | EUR 1633 |
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Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
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OxiSelect 96-Well Comet Assay Slide |
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| STA-356 | Cell Biolabs | 1 slide | EUR 183 |
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Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
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OxiSelect 96-Well Comet Assay Slide |
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| STA-356-5 | Cell Biolabs | 5 slides | EUR 519 |
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Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
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OxiSelect BPDE DNA Adduct ELISA Kit |
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| STA-357 | Cell Biolabs | 96 assays | EUR 757 |
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Description: BPDE-DNA standards or unknown DNA samples are adsorbed onto a 96-well DNA high-binding plate. The BPDE-DNA adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody. The BPDE-DNA adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-DNA standards. |
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OxiSelect Methylglyoxal (MG) Competitive ELISA Kit |
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| STA-811 | Cell Biolabs | 96 assays | EUR 798 |
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Description: The OxiSelect Methylglyoxal (MG) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts. The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples. |
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OxiSelect Methylglyoxal (MG) Competitive ELISA Kit |
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| STA-811-5 | Cell Biolabs | 5 x 96 assays | EUR 3240 |
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Description: The OxiSelect Methylglyoxal (MG) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts. The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples. |
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OxiSelect MDA Adduct Competitive ELISA Kit |
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| STA-832 | Cell Biolabs | 96 assays | EUR 798 |
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Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Competitive ELISA Kit provides a sensitive, specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE. |
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OxiSelect MDA Adduct Competitive ELISA Kit |
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| STA-832-5 | Cell Biolabs | 5 x 96 assays | EUR 3240 |
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Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Competitive ELISA Kit provides a sensitive, specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE. |
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OxiSelect HNE Adduct Competitive ELISA Kit |
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| STA-838 | Cell Biolabs | 96 assays | EUR 798 |
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Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format. |
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OxiSelect HNE Adduct Competitive ELISA Kit |
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| STA-838-5 | Cell Biolabs | 5 x 96 assays | EUR 3240 |
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Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format. |
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OxiSelect Ascorbic Acid Assay Kit (FRASC) |
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| STA-860 | Cell Biolabs | 200 assays | EUR 560 |
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Description: Ascorbic acid is a vital water-soluble antioxidant found in living organisms. Ascorbic acid is critical for a variety of functions related to tissue growth and wound healing, neurotransmitter formation, blood cholesterol levels, as well as free radical neutralization. The OxiSelect Ascorbic Acid Assay Kit is a quantitative assay for measuring ascorbic acid within various samples. The assay is based on the Ferric Reducing/Antioxidant Ascorbic Acid (FRASC) chemistry driven by the electron donating reducing power of antioxidants. The kit employs ascorbate oxidase, which allows the user to differentiate the ascorbic acid content from other antioxidants present within their sample. Ascorbic acid levels in a sample are determined by measuring the difference in optical density between two sample wells, one with and one without the enzyme. |
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OxiSelect Protein Carbamylation Sandwich ELISA Kit |
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| STA-877 | Cell Biolabs | 96 assays | EUR 780 |
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Description: The OxiSelect Protein Carbamylation Sandwich ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of protein carbamylation. The quantity of carbamylated adduct in protein samples is determined by comparing its absorbance with that of a known CBL-BSA standard curve. The kit has a detection sensitivity limit of 1.5 ng/mL of CBL-BSA. |
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OxiSelect Monoamine Oxidase Assay Kit (Fluorometric) |
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| XPX-5000 | Cell Biolabs | 96 assays | EUR 450 |
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Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected fluorescence plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve. |
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OxiSelect Monoamine Oxidase Assay Kit (Fluorometric) |
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| XPX-5000-5 | Cell Biolabs | 5 x 96 assays | EUR 1795 |
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Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected fluorescence plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve. |
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OxiSelect Monoamine Oxidase Assay Kit (Colorimetric) |
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| XPX-5006 | Cell Biolabs | 96 assays | EUR 450 |
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Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected colorimetric plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve. |
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OxiSelect Monoamine Oxidase Assay Kit (Colorimetric) |
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| XPX-5006-5 | Cell Biolabs | 5 x 96 assays | EUR 1795 |
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Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected colorimetric plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve. |
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OxiSelect Protein Carbonyl Fluorometric Assay, Trial Size |
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| STA-307-T | Cell Biolabs | 20 assays | EUR 351 |
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Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Fluorometric Assay Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The fluorescence plate-based format provides a convenient system for direct measurement of protein carbonyl content. |
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OxiSelect Protein Carbonyl Immunoblot Kit, Trial Size |
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| STA-308-T | Cell Biolabs | 4 blots | EUR 264 |
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Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Immunoblot Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The immunoblot format provides a convenient way to compare oxidized and non-oxidized protein fingerprints. |
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OxiSelect Protein Carbonyl ELISA Kit (1 plate) |
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| STA-310 | Cell Biolabs | 96 assays | EUR 798 |
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Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl ELISA Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The ELISA format is perfect for higher throughput and high sensitivity; we have eliminated concentration and precipitation steps allowing greater sample retention. |
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OxiSelect Protein Carbonyl ELISA Kit (5 plates) |
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| STA-310-5 | Cell Biolabs | 5 x 96 assays | EUR 3240 |
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Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl ELISA Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The ELISA format is perfect for higher throughput and high sensitivity; we have eliminated concentration and precipitation steps allowing greater sample retention. |
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OxiSelect Protein Carbonyl ELISA Kit, Trial Size |
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| STA-310-T | Cell Biolabs | 32 assays | EUR 421 |
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Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl ELISA Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The ELISA format is perfect for higher throughput and high sensitivity; we have eliminated concentration and precipitation steps allowing greater sample retention. |
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OxiSelect Total Glutathione (GSSG/GSH) Assay Kit |
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| STA-312 | Cell Biolabs | 100 assays | EUR 519 |
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Description: The OxiSelect Total Glutathione Assay Kit is a quantitative assay for measuring the total glutathione content within a sample (GSH/GSSG). Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH) in the presence of NADPH. Subsequently, the chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm. The total glutathione content in unknown samples is determined by comparison with the predetermined glutathione standard curve. The rate of chromophore production is proportional to the concentration of glutathione within the sample. The rate can be determined from the absorbance change over time. Metaphosphoric acid is provided to remove interfering proteins or enzymes from samples. |
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OxiSelect 8-iso-Prostaglandin F2a ELISA Kit |
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| STA-337 | Cell Biolabs | 96 assays | EUR 606 |
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Description: 8-iso-Prostaglandin F2alpha (8-isoprostane) is a stable by-product of lipid peroxides generated during oxidative stress. Our OxiSelect 8-Isoprostane Assay Kit provides a convenient method for absolute quantitation in just a few hours. The 8-Isoprostane ELISA is suitable for a variety of sample types including urine, plasma, and cell lysates. |
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OxiSelect 8-iso-Prostaglandin F2a ELISA Kit |
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| STA-337-5 | Cell Biolabs | 5 x 96 assays | EUR 2428 |
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Description: 8-iso-Prostaglandin F2alpha (8-isoprostane) is a stable by-product of lipid peroxides generated during oxidative stress. Our OxiSelect 8-Isoprostane Assay Kit provides a convenient method for absolute quantitation in just a few hours. The 8-Isoprostane ELISA is suitable for a variety of sample types including urine, plasma, and cell lysates. |
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OxiSelect Superoxide Dismutase Activity Assay, Trial Size |
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| STA-340-T | Cell Biolabs | 20 assays | EUR 345 |
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Description: Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidant enzymes. SOD enzymes are classified into three groups: cytosolic CuZn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our OxiSelect Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions and a chromagen to produce a water-soluble formazan dye upon reduction by superoxide anions. The superoxide dismutase activity is determined as the inhibition or reduction of chromagen. |
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OxiSelect Intracellular ROS Assay Kit (Green Fluorescence) |
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| STA-342 | Cell Biolabs | 96 assays | EUR 635 |
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Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader. |
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OxiSelect Intracellular ROS Assay Kit (Green Fluorescence) |
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| STA-342-5 | Cell Biolabs | 5 x 96 assays | EUR 2549 |
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Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader. |
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OxiSelect Hydrogen Peroxide / Peroxidase Assay Kit (Fluorometric) |
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| STA-344 | Cell Biolabs | 500 assays | EUR 566 |
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Description: The OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit is a sensitive quantitative fluorometric assay for hydrogen peroxide or peroxidase. In the presence of HRP, ADHP reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin. The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm, or for absorbance at 560 nm. Fluorescence values are proportional to the H2O2 or peroxidase levels within the samples. The H2O2 or peroxidase content in unknown samples is determined by comparison with its respective standard curve. |
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OxiSelect Cellular Antioxidant Assay Kit (Green Fluorescence) |
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| STA-349 | Cell Biolabs | 192 assays | EUR 537 |
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Description: The OxiSelect Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells. Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner. |
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OxiSelect Comet Assay Kit (3-Well Slides) |
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| STA-350 | Cell Biolabs | 15 assays | EUR 328 |
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Description: Our OxiSelect 3-Well Comet Assay Kits provide a fast, convenient way to screen for general DNA damage, regardless of the source or nature of the damage. Kits include Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
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OxiSelect Comet Assay Kit (3-Well Slides) |
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| STA-351 | Cell Biolabs | 75 assays | EUR 601 |
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Description: Our OxiSelect 3-Well Comet Assay Kits provide a fast, convenient way to screen for general DNA damage, regardless of the source or nature of the damage. Kits include Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
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OxiSelect Comet Assay Kit (3-Well Slides) |
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| STA-351-5 | Cell Biolabs | 5 x 75 assays | EUR 2364 |
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Description: Our OxiSelect 3-Well Comet Assay Kits provide a fast, convenient way to screen for general DNA damage, regardless of the source or nature of the damage. Kits include Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
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OxiSelect Total Antioxidant Capacity (TAC) Assay Kit |
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| STA-360 | Cell Biolabs | 200 assays | EUR 519 |
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Description: The OxiSelect Total Antioxidant Capacity (TAC) Assay measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. In the presence of antioxidants, copper(II) is reduced to copper(I). In turn, the copper(I) ions react with a chromogen to produce a color with maximum absorbance at 490nm. |
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OxiSelect Myeloperoxidase Chlorination Activity Assay Kit (Colorimetric) |
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| STA-803 | Cell Biolabs | 200 assays | EUR 554 |
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Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, colorimetric assay for measuring the myeloperoxidase activity within a sample. |
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OxiSelect Myeloperoxidase Chlorination Activity Assay Kit (Fluorometric) |
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| STA-804 | Cell Biolabs | 192 assays | EUR 577 |
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Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase activity within a sample. |
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OxiSelect Myeloperoxidase Peroxidation Activity Assay Kit (Fluorometric) |
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| STA-805 | Cell Biolabs | 192 assays | EUR 577 |
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Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase peroxidation activity within a sample. |
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OxiSelect s-Glutathione Adduct Competitive ELISA Kit |
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| STA-814 | Cell Biolabs | 96 assays | EUR 624 |
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Description: Glutathione helps protect cells from free radical damage as an antioxidant, but it can also covalently attach to proteins through its sulfhydryl moiety upon derivatization by reactive oxygen species (ROS). Glutathione protein adducts can therefore serve as a marker of oxidative stress. OxiSelect s-Glutathione Adduct Competitive ELISA Kit quantifies the glutathionylation of proteins in a standard 96-well plate. |
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OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit (Colorimetric) |
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| STA-844 | Cell Biolabs | 500 assays | EUR 531 |
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Description: The OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit is a sensitive colorimetric assay for hydrogen peroxide or peroxidase. The H2O2 or peroxidase content in unknowns is compared with a standard curve. 500 assays/kit. |
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OxiSelect Free Hydrogen Sulfide Gas Assay Kit |
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| XAN-5084 | Cell Biolabs | 100 assays | EUR 427 |
OxiSelect N-epsilon-(Carboxymethyl) Lysine (CML) Immunoblot Kit |
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| STA-313 | Cell Biolabs | 10 blots | EUR 531 |
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Description: Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The OxiSelect N-epsilon-(Carboxymethyl) Lysine Immunoblot kit specifically detects CML formation in protein samples. |
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OxiSelect DNA Double-Strand Break (DSB) Staining Kit |
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| STA-321 | Cell Biolabs | 100 assays | EUR 659 |
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Description: Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect DNA Double-Strand Break Staining Kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX. |
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OxiSelect Oxidative DNA Damage Quantitation Kit (AP sites) |
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| STA-324 | Cell Biolabs | 50 assays | EUR 780 |
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Description: DNA damage can manifest in the formation of apurinic or apyrimidinic (AP or abasic) sites. Such spontaneous base loss in mammalian cells has been estimated at between 50,000 and 200,000 sites per day. Unrepaired abasic sites can inhibit transcription and may be mutagenic. Our OxiSelect Oxidative DNA Damage Quantitation Kit provides a simple, user-friendly method for the quantitaiton DNA damage in the form of abasic sites. The assay uses an Aldehyde Reactive Probe (ARP) to specifically react with an aldehyde group on the open ring of the AP site. This allows the AP site to be labeled with Biotin, followed by detection with Streptavidin-Enzyme conjugate. |
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OxiSelect TBARS Assay Kit (MDA Quantitation), Trial Size |
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| STA-330-T | Cell Biolabs | 20 assays | EUR 340 |
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Description: The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE. |
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OxiSelect Catalase Activity Assay Kit, Fluorometric, Trial Size |
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| STA-339-T | Cell Biolabs | 50 assays | EUR 398 |
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Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kit measures catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 500 assays in a 96-well microtiter plate. This includes blanks, catalase standards and unknown samples. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay. |
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OxiSelect Catalase Activity Assay Kit (Colorimetric), Trial Size |
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| STA-341-T | Cell Biolabs | 20 assays | EUR 363 |
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Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kits measure catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 100 assays in a 96-well microtiter plate, or 50 assays in microcentrifuge tubes. This includes blanks, catalase standards and unknown samples. Direct spectrophotometric detection of catalase activity with ultraviolet light can cause interference from proteins and other biological components. The OxiSelect Catalase Activity Assay Kit (Colorimetric) utilizes visible light (520 nm), which reduces sample interference. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay. |
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OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric) |
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| STA-800 | Cell Biolabs | 96 assays | EUR 490 |
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Description: The OxiSelect Intracellular Nitric Oxide Assay Kit is a cell-based assay for rapid detection of intracellular NO or NOS activity in cultured cells. In brief, the cell-permeant NO probe passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate. When intracellular nitric oxide encounters this intermediate, it rapidly oxidizes to a highly fluorescent, triazolo-fluorescein analog. The fluorescence intensity is proportional to the NO levels within the cell cytosol (detection limit of ~3 nM). The kit?s provided Nitric Oxide Fluorometric Probe is NO specific, has excellent photostablity and pH stability, and is detected with standard fluorescein filters. The probe is suitable for flow cytometry, fluorescent microscopy, and fluorescent microplate detection. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards, and unknown samples. |
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OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric) |
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| STA-800-5 | Cell Biolabs | 5 x 96 assays | EUR 1923 |
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Description: The OxiSelect Intracellular Nitric Oxide Assay Kit is a cell-based assay for rapid detection of intracellular NO or NOS activity in cultured cells. In brief, the cell-permeant NO probe passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate. When intracellular nitric oxide encounters this intermediate, it rapidly oxidizes to a highly fluorescent, triazolo-fluorescein analog. The fluorescence intensity is proportional to the NO levels within the cell cytosol (detection limit of ~3 nM). The kit?s provided Nitric Oxide Fluorometric Probe is NO specific, has excellent photostablity and pH stability, and is detected with standard fluorescein filters. The probe is suitable for flow cytometry, fluorescent microscopy, and fluorescent microplate detection. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards, and unknown samples. |
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OxiSelect N?-(carboxyethyl) lysine (CEL) Competitive ELISA Kit |
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| STA-813 | Cell Biolabs | 96 assays | EUR 798 |
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Description: First, a CEL conjugate is coated on the ELISA plate. The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate. After a brief incubation, the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CEL protein adducts in unknown samples is determined by comparison with the predetermined CEL-BSA standard curve. Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in the OxiSelect CEL ELISA Kit will not cross react with CML protein adducts. |
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OxiSelect N?-(carboxymethyl) lysine (CML) Competitive ELISA Kit |
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| STA-816 | Cell Biolabs | 96 assays | EUR 798 |
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Description: Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The OxiSelect N-epsilon-(Carboxymethyl) Lysine Competitive ELISA kit specifically detects CML formation with a high level of sensitivity. This is a Competitive ELISA Kit in which the plate is coated with a CML conjugate. Standards and unknown samples are added to the plate, followed by incubation with the primary antibody. The CML in the unknown samples and the CML attached to the plate compete for the primary antibody. Higher CML content in unknown samples results in more binding of the antibody to the sample, and thus less antibody binds to the plate. Since the antibody bound to the sample is washed away, higher CML content in samples correlates with a lower signal. |
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OxiSelect N?-(carboxymethyl) lysine (CML) Competitive ELISA Kit |
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| STA-816-5 | Cell Biolabs | 5 x 96 assays | EUR 3240 |
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Description: Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The OxiSelect N-epsilon-(Carboxymethyl) Lysine Competitive ELISA kit specifically detects CML formation with a high level of sensitivity. This is a Competitive ELISA Kit in which the plate is coated with a CML conjugate. Standards and unknown samples are added to the plate, followed by incubation with the primary antibody. The CML in the unknown samples and the CML attached to the plate compete for the primary antibody. Higher CML content in unknown samples results in more binding of the antibody to the sample, and thus less antibody binds to the plate. Since the antibody bound to the sample is washed away, higher CML content in samples correlates with a lower signal. |
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OxiSelect MDA Adduct Competitive ELISA Kit, Trial Size |
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| STA-832-T | Cell Biolabs | 32 assays | EUR 421 |
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Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Competitive ELISA Kit provides a sensitive, specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE. |
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OxiSelect HNE Adduct Competitive ELISA Kit, Trial Size |
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| STA-838-T | Cell Biolabs | 32 assays | EUR 421 |
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Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format. |
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OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit |
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| STA-859 | Cell Biolabs | 200 assays | EUR 508 |
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Description: OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit is a quantitative assay for measuring the antioxidant potential within various samples. Following the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+) by antioxidants present in the sample, the kit colorimetric probe develops a blue color that is read colorimetrically at 540-600nm. The antioxidant potential of samples is determined based on an iron standard curve and results are calculated at Fe2+ equivalents (µM) or FRAP value. |
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OxiSelect Total Glutathione (GSSG/GSH) Assay Kit, Trial Size |
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| STA-312-T | Cell Biolabs | 20 assays | EUR 282 |
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Description: The OxiSelect Total Glutathione Assay Kit is a quantitative assay for measuring the total glutathione content within a sample (GSH/GSSG). Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH) in the presence of NADPH. Subsequently, the chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm. The total glutathione content in unknown samples is determined by comparison with the predetermined glutathione standard curve. The rate of chromophore production is proportional to the concentration of glutathione within the sample. The rate can be determined from the absorbance change over time. Metaphosphoric acid is provided to remove interfering proteins or enzymes from samples. |
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OxiSelect UV-Induced DNA Damage ELISA Kit (CPD Quantitation) |
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| STA-322 | Cell Biolabs | 96 assays | EUR 659 |
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Description: Our OxiSelect UV-Induced DNA Damage ELISA Kit measures the formation of cyclobutane pyrimidine dimers (CPD) in DNA isolated from cells or tissues. Standards or unknown DNA samples are first heat denatured before adsorbed onto a 96-well DNA binding plate. The sample or standard are then probed with an anti-CPD antibody, followed by an HRP conjugated secondary antibody. |
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OxiSelect UV-Induced DNA Damage ELISA Kit (CPD Quantitation) |
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| STA-322-5 | Cell Biolabs | 5 x 96 assays | EUR 2671 |
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Description: Our OxiSelect UV-Induced DNA Damage ELISA Kit measures the formation of cyclobutane pyrimidine dimers (CPD) in DNA isolated from cells or tissues. Standards or unknown DNA samples are first heat denatured before adsorbed onto a 96-well DNA binding plate. The sample or standard are then probed with an anti-CPD antibody, followed by an HRP conjugated secondary antibody. |
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OxiSelect Cellular UV-Induced DNA Damage ELISA Kit (CPD) |
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| STA-326 | Cell Biolabs | 96 assays | EUR 659 |
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Description: Our OxiSelect Cellular UV-Induced DNA Damage ELISA Kit mesaures the formation of cyclobutane pyrimidine dimers (CPD) in intact cells. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by an HRP conjugated secondary antibody. |
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OxiSelect Cellular UV-Induced DNA Damage ELISA Kit (CPD) |
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| STA-326-5 | Cell Biolabs | 5 x 96 assays | EUR 2671 |
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Description: Our OxiSelect Cellular UV-Induced DNA Damage ELISA Kit mesaures the formation of cyclobutane pyrimidine dimers (CPD) in intact cells. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by an HRP conjugated secondary antibody. |
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OxiSelect Cellular UV-Induced DNA Damage Staining Kit (CPD) |
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| STA-327 | Cell Biolabs | 96 assays | EUR 659 |
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Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope. |
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OxiSelect 8-iso-Prostaglandin F2a ELISA Kit, Trial Size |
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| STA-337-T | Cell Biolabs | 32 assays | EUR 328 |
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Description: 8-iso-Prostaglandin F2alpha (8-isoprostane) is a stable by-product of lipid peroxides generated during oxidative stress. Our OxiSelect 8-Isoprostane Assay Kit provides a convenient method for absolute quantitation in just a few hours. The 8-Isoprostane ELISA is suitable for a variety of sample types including urine, plasma, and cell lysates. |
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OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size |
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| STA-342-T | Cell Biolabs | 20 assays | EUR 345 |
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Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader. |
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OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit (Fluorometric), Trial Size |
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| STA-344-T | Cell Biolabs | 50 assays | EUR 287 |
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Description: The OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit is a sensitive quantitative fluorometric assay for hydrogen peroxide or peroxidase. In the presence of HRP, ADHP reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin. The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm, or for absorbance at 560 nm. Fluorescence values are proportional to the H2O2 or peroxidase levels within the samples. The H2O2 or peroxidase content in unknown samples is determined by comparison with its respective standard curve. |
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OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence) |
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| STA-347 | Cell Biolabs | 96 assays | EUR 612 |
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Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF. |
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OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence) |
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| STA-347-5 | Cell Biolabs | 5 x 96 assays | EUR 2428 |
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Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF. |
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OxiSelect Human Oxidized LDL ELISA Kit (OxPL-LDL Quantitation) |
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| STA-358 | Cell Biolabs | 96 assays | EUR 780 |
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Description: LDL, so-called "bad cholesterol", is even more dangerous when it becomes oxidized. Oxidized LDL (OxLDL) is more reactive with surrounding tissues and can collect within the inner-lining of arteries. Macrophages, cholesterol, and other lipids can accumulate at the site (atherosclerosis), ultimately forming a plaque that can lead to heart attack, stroke or death. Oxidation of LDL affects both the lipid and protein components of LDL. The OxiSelect Human Oxidized LDL ELISA Kits are enzyme immunoassays developed for the detection and quantitation of human oxLDL in plasma, serum or other biological fluid samples. The kits contain a copper oxidized LDL standard against which unknown samples may be compared. This oxidized LDL assay kit is configured to selectively measure OxLDL in the phospholipid component (OxPL). |
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OxiSelect Total Antioxidant Capacity (TAC) Assay Kit, Trial Size |
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| STA-360-T | Cell Biolabs | 20 assays | EUR 282 |
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Description: The OxiSelect Total Antioxidant Capacity (TAC) Assay measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. In the presence of antioxidants, copper(II) is reduced to copper(I). In turn, the copper(I) ions react with a chromogen to produce a color with maximum absorbance at 490nm. |
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OxiSelect Human Oxidized LDL ELISA Kit (MDA-LDL Quantitation) |
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| STA-369 | Cell Biolabs | 96 assays | EUR 780 |
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Description: LDL, so-called "bad cholesterol", is even more dangerous when it becomes oxidized. Oxidized LDL (OxLDL) is more reactive with surrounding tissues and can collect within the inner-lining of arteries. Macrophages, cholesterol, and other lipids can accumulate at the site (atherosclerosis), ultimately forming a plaque that can lead to heart attack, stroke or death. Oxidation of LDL affects both the lipid and protein components of LDL. The OxiSelect Human Oxidized LDL ELISA Kits are enzyme immunoassays developed for the detection and quantitation of human oxLDL in plasma, serum or other biological fluid samples. The kits contain a copper oxidized LDL standard against which unknown samples may be compared. This oxidized LDL assay kit is configured to selectively measure oxLDL in MDA-LDL. |
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OxiSelect Human Oxidized LDL ELISA Kit (CML-LDL Quantitation) |
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| STA-388 | Cell Biolabs | 96 assays | EUR 780 |
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Description: LDL, so-called "bad cholesterol", is even more dangerous when it becomes oxidized. Oxidized LDL (OxLDL) is more reactive with surrounding tissues and can collect within the inner-lining of arteries. Macrophages, cholesterol, and other lipids can accumulate at the site (atherosclerosis), ultimately forming a plaque that can lead to heart attack, stroke or death. Oxidation of LDL affects both the lipid and protein components of LDL. The OxiSelect Human Oxidized LDL ELISA Kits are enzyme immunoassays developed for the detection and quantitation of human oxLDL in plasma, serum or other biological fluid samples. The kits contain a copper oxidized LDL standard against which unknown samples may be compared. This oxidized LDL assay kit is configured to selectively measure oxLDL in CML-LDL. |
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OxiSelect Human Oxidized LDL ELISA Kit (HNE-LDL Quantitation) |
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| STA-389 | Cell Biolabs | 96 assays | EUR 780 |
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Description: LDL, so-called "bad cholesterol", is even more dangerous when it becomes oxidized. Oxidized LDL (OxLDL) is more reactive with surrounding tissues and can collect within the inner-lining of arteries. Macrophages, cholesterol, and other lipids can accumulate at the site (atherosclerosis), ultimately forming a plaque that can lead to heart attack, stroke or death. Oxidation of LDL affects both the lipid and protein components of LDL. The OxiSelect Human Oxidized LDL ELISA Kits are enzyme immunoassays developed for the detection and quantitation of human oxLDL in plasma, serum or other biological fluid samples. The kits contain a copper oxidized LDL standard against which unknown samples may be compared. This oxidized LDL assay kit is configured to selectively measure oxLDL in HNE-LDL. |
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OxiSelect Advanced Glycation End Product (AGE) Competitive ELISA Kit |
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| STA-817 | Cell Biolabs | 96 assays | EUR 740 |
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Description: Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins (Figure 1). The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent advanced glycation end products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. Our OxiSelect AGE Competitive ELISA kit is designed for rapid detection and quantitation of advanced glycation end product protein adducts. The quantity of AGE adduct in protein samples is determined by comparing its OD with that of a known AGE-BSA standard curve. |
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OxiSelect Advanced Glycation End Product (AGE) Competitive ELISA Kit |
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| STA-817-5 | Cell Biolabs | 5 x 96 assays | EUR 3025 |
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Description: Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins (Figure 1). The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent advanced glycation end products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. Our OxiSelect AGE Competitive ELISA kit is designed for rapid detection and quantitation of advanced glycation end product protein adducts. The quantity of AGE adduct in protein samples is determined by comparing its OD with that of a known AGE-BSA standard curve. |
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OxiSelect Aldehyde-Induced DNA Damage ELISA Kit (Ethenoadenosine Quantitation) |
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| STA-820 | Cell Biolabs | 96 assays | EUR 635 |
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Description: The OxiSelect Aldehyde-Induced DNA Damage ELISA Kit (Ethenoadenosine Quantitation) is a competitive ELISA kit allowing the quantitation of ethenoadenosine adducts in DNA isolated from cells or tissues. Samples and standards are added to a provided microplate preabsorbed with etheno-damaged DNA. An anti-ethenoadenosine antibody is added and binds competitively to ethenoadenosine in the unknown sample or to the ethenoadenosine in the DNA coated on the plate. After washing a secondary antibody is added. A low signal means most of the primary antibody was bound to the unknown, indicating a high level of ethenoadenosine in the sample. |
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OxiSelect Aldehyde-Induced DNA Damage ELISA Kit (Ethenocytidine Quantitation) |
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| STA-821 | Cell Biolabs | 96 assays | EUR 635 |
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Description: The OxiSelect Aldehyde-Induced DNA Damage ELISA Kit (Ethenocytidine Quantitation) is a competitive ELISA kit allowing the quantitation of ethenocytidine adducts in DNA isolated from cells or tissues. Samples and standards are added to a provided microplate preabsorbed with etheno-damaged DNA. An anti-ethenocytidine antibody is added and binds competitively to ethenocytidine in the unknown sample or to the ethenocytidine in the DNA coated on the plate. After washing a secondary antibody is added. A low signal means most of the primary antibody was bound to the unknown, indicating a high level of ethenocytidine in the sample. |
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New checks have the potential to enhance accuracy, operational traits and end-user entry to checks for TB an infection. Nonetheless, printed knowledge in varied populations and settings are restricted for many new checks. Analysis of those new checks in a standardised design would facilitate their endorsement and programmatic scale-up. Nonetheless, each sorts of checks have restricted efficiency to foretell growth of energetic TB.