RRM2 silencing suppresses malignant phenotype and enhances radiosensitivity via activating cGAS/STING signaling pathway in lung adenocarcinoma

RRM2 silencing suppresses malignant phenotype and enhances radiosensitivity via activating cGAS/STING signaling pathway in lung adenocarcinoma

Posted on by Antonio

As one of the widespread malignancy, lung adenocarcinoma (LUAD) is characterised by low 5-year survival price. This analysis aimed to analyze the results of ribonucleotide reductase regulatory subunit M2 (RRM2) on malignant organic behaviors and activation of cGAS/STING pathway. We additionally explored the synergistic sensitization mechanisms of RRM2 and radiotherapy. Bioinformatic instruments have been used to guage the medical significance of RRM2 in LUAD sufferers. The roles of RRM2 in malignant phenotype and DNA injury in LUAD cells have been investigated with cell proliferation, colony formation, immunofluorescence, modified Boyden chamber and comet assays.

The mouse fashions have been used to guage the organic significance of RRM2 in vivo. Cytotoxic T cell infiltration was evaluated through circulation cytometric evaluation and immunohistochemistry staining in C57BL/6 mice. We additionally explored the synergistic results of RRM2 silencing and radiation on LUAD cells with apoptosis assay and immunoblotting in vitro. Bioinformatic evaluation revealed that RRM2 had diagnostic values for LUAD sufferers. Larger ranges of RRM2 predicted worse prognosis. RRM2 silencing inhibited LUAD cell proliferation, invasion and migration.

RRM2 knockdown induced S section arrest and DNA injury. RRM2 silencing induced cyclic GMP-AMP synthase (cGAS)/stimulator of interferon genes (STING) pathway, and the downstream targets have been regulated in a STING-dependent method. Knockdown of RRM2 suppressed tumor progress within the xenograft tumor fashions. RRM2 deficiency elevated CD8 + T cells within the tumor tissues and spleens. Moreover, RRM2 silencing had synergistic results with radiation on inhibiting cell proliferation and selling apoptosis. In the meantime, this mixture promoted the activation of cGAS/STING signaling pathway synergistically, and concurrently elevated expression of IFNβ, CCL5 and CXCL10.

Our outcomes demonstrated that RRM2 silencing had anti-tumor values and activated the cGAS/STING signaling pathway. RRM2 silencing elevated CD8 + T cells infiltration. RRM2 silencing cooperated with radiation to inhibit LUAD cell proliferation, promote apoptosis and improve the activation of cGAS/STING signaling pathway. RRM2 could possibly be a promising goal for tumor regression by most cancers immunotherapy in LUAD.

Identification of the canonical and noncanonical position of miR-143/145 in estrogen-deficient bone loss

Postmenopausal-induced bone loss is especially brought on by declining core transcription elements (TFs) of bone mesenchymal stem cells (BMSCs), however little is thought about how miRNAs regulate chromatin construction reworking of TFs gene to keep up BMSCs perform in bone homeostasis. We examined the serum, salivary and bone samples from Pre- and Submit-menopause ladies by paired evaluation and confirmed canonical ceRNA position of MIR143HG and miR-143/145 complexes in cytoplasm and noncanonical position for SOX2 transcription in nucleus (FISH, qRT-PCR, immunostaining, Luciferase assays and ChIP).

Furthermore, we took benefit of transgenic mice underneath OVX-induced osteoporosis, learning the in vitro and in vivo impact of miR-143/145 deletion on BMSCs perform and bone homeostasis. Final, utilizing miRNA antagonism, antagomiR-143/145 have been delivered into bone marrow to deal with estrogen-deficient bone loss. Right here, we recognized miR-143/145 as potential diagnostic candidates for postmenopausal osteoporosis, and miR-143/145 overexpression impaired BMSCs self-renewing and differentiation perform.

Mechanistically, we confirmed that cytoplasmic miR-143/145 and LncRNA MIR143HG, that managed by ERβ, cooperatively regulated pluripotency genes translation through canonical ceRNA pathway, and MIR143HG cooperates with miR‑143 to nuclear translocation for co-activation of SOX2 transcription through opening promoter chromatin. In the meantime, miR‑143/145 have been shuttled into osteoclasts in extracellular vesicles and triggered osteoclastic exercise by focusing on Cd226 and Srgap2.

Moreover, miR-143/145-/- mice or utilizing chemically‑modified antagomiR-143/145 considerably alleviated estrogen-deficient osteoporosis. Our findings reveal a canonical and noncanonical position of miR-143/145 in controlling BMSCs pluripotency and unfold their twin impact on bone formation and bone resorption, suggesting miR-143/145 as promising therapeutic targets for treating estrogen-deficient bone loss. Present diagnostics for Valley fever are severely missing resulting from poor sensitivity and invasiveness, contributing to a 23-day median time to analysis, and subsequently, new diagnostic instruments are wanted.

RRM2 silencing suppresses malignant phenotype and enhances radiosensitivity via activating cGAS/STING signaling pathway in lung adenocarcinoma

MiR-34b-3p Impaired HUVECs Viability and Migration through Concentrating on PDK1 in an In Vitro Mannequin of Gestational Diabetes Mellitus

Gestational diabetes mellitus (GDM) results in poor being pregnant outcomes. The strategies for GDM early analysis and remedy are nonetheless unknown. This research aimed to analyze the expression and diagnostic potential of miR-34b-3p in GDM sufferers and additional analyzed the results of miR-34b-3p on HUVECs viability and migration. The expression of miR-34b-3p was detected in HUVECs of GDM and regular pregnant ladies by qRT-PCR. Then the HUVECs have been remoted from regular pregnant ladies. Excessive glucose (HG) was used to deal with the HUVECs to imitate the GDM in vitro.

The cell viability and migration have been decided by MTT, wound therapeutic assay, and transwell assay. The interplay between miR-34b-3p and PDK1 was evaluated by luciferase exercise assay. Our outcomes confirmed that miR-34b-3p was up-regulated in HUVECs of GDM sufferers. Then the HUVECs have been remoted from regular pregnant ladies they usually have been handled with HG to imitate the GDM in vitro. Curiously, knockdown of miR-34b-3p restored the impairment of HG treatment-induced results in HUVECs. Extra importantly, PDK1 was proved to be a possible goal of miR-34b-3p.

Phosphate Assay Kit (Fluorometric)
K420-100 each
EUR 418.8

Phosphate Assay Kit (Fluorometric)
STA-686 1000 assays
EUR 582
Description: Our Phosphate Assay Kit (Fluorometric) measures total inorganic phosphate present in lysates, solutions, food, or biological samples in a 96-well fluorescence-based plate reader. 1000 assays/kit.

PiBlue™ Phosphate Assay Kit
POPB-500 500
EUR 239
Description: For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 20 pmoles of phosphate and useful range between 0.4 µM and 50 µM phosphate. Fast and convenient: single reagent "mix-and-measure" assay allows quantitation of free phosphate within 30 minutes. Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well plates. Can be readily automated on HTS liquid handling systems Method: OD620nm (malachite green). Samples: Enzyme reactions with free phosphate releases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 0.4 µM.

QuantiChrom™ Phosphate Assay Kit
DIPI-500 500
EUR 349
Description: For quantitative determination of phosphate and evaluation of drug effects on phosphate metabolism. Key Features: Sensitive and accurate. Linear detection range 0.3 µM (0.0028 mg/dL) to 50 µM (0.47 mg/dL) phosphate in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in cuvet or 96-well plate. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals. Method: OD620nm (malachite green). Samples: Serum, urine, saliva, sweat, tissue culture, food, environment etc. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 3 µg/dL (0.3 µM).

Malachite Green Phosphate Assay kit
65-POMG-25H 2500 tests
EUR 333

Malachite Green Phosphate Assay Kit
abx298878-100Assays 100 Assays
EUR 453.6

Malachite Green Phosphate Assay Kit
POMG-25H 2500
EUR 259
Description: For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 1.6 pmoles of phosphate and useful range between 0.02 µM and 40 µM phosphate. Fast and convenient: homogeneous "mix-and-measure" assay allows quantitation of free phosphate within 20 minutes. Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: OD620nm (malachite green). Samples: Biological, environment etc. Species: All. Procedure: Assay takes 30 min. Kit size: 2500 tests. Detection limit: 0.02 µM.

Malachite Green Phosphate Assay Kit
Z5030012 2,500 assays
EUR 312

Phosphate Fluorometric Assay Kit
55R-1404 100 assays
EUR 525.6
Description: Assay Kit for detection of Phosphate in the research laboratory

Phosphate Colorimetric Assay Kit
k2074-500 500 assays
EUR 351.6

Phosphate Colorimetric Assay Kit
K410-500 each
EUR 379.2

Sucrose Phosphate Synthase Assay Kit
abx298805-100Assays 100 Assays
EUR 754.8

MESG *Phosphate assay reagent*
21600 5 mg
EUR 199

MESG *Phosphate assay reagent*
21600-1MG 1 mg
EUR 77

MESG *Phosphate assay reagent*
21600-1MG-1mg 1 mg
EUR 77
Description: In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red.

MESG *Phosphate assay reagent*
21600-5mg 5 mg
EUR 199
Description: In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red.

Phosphoprotein Phosphate Estimation Assay Kit
BSP061 2000Assays, 2000preps
EUR 110.11

PicoProbe? Phosphate Fluorometric Assay Kit
K419-100 each
EUR 535.2

MicroMolar Phosphate Assay Reagent
MPA3000 3000 assays
EUR 168.93
Description: This product includes 450 ml of reagent. It is for 3000 to 5000 assays using 96-well plates or more than 10,000 assays using 384-well plates. It can also be used for measurement of phosphate concentrations using cuvettes and a spectrophotometer.

PhosphoWorksâ„¢ Colorimetric Phosphate Assay Kit *Blue Color*
21665-1000Tests 1000 Tests
EUR 222
Description: Phosphate is involved in many biological reactions.

Phosphate Assay, Catalog: MA-0105
MA-0105 100 wells
EUR 255

PhosphoWorksâ„¢ Fluorimetric Phosphate Assay Kit *Red Fluorescence*
21660-125Tests 125 Tests
EUR 334
Description: Cells utilize a wide variety of phosphate (Pi) and polyphosphate esters as enzyme substrates, second messengers, membrane structural components and vital energy reservoirs.

PhosphoWorksâ„¢ Fluorimetric Phosphate Assay Kit *Red Fluorescence*
21661-1250Tests 1250 Tests
EUR 2197
Description: Cells utilize a wide variety of phosphate (Pi) and polyphosphate esters as enzyme substrates, second messengers, membrane structural components and vital energy reservoirs.

Malachite Green Phosphate Microplate Assay Kit
DLSM0112 100 Assays
EUR 262.5
Description: Detection and Quantification of Phosphate Content.

Sucrose Phosphate Synthase Microplate Assay Kit
DLSM0039 100 Assays
EUR 595
Description: Detection and Quantification of Sucrose Phosphate Synthase Activity.

PhosphoWorks™ Colorimetric Phosphate Assay Kit *Blue Color*
21665 1000 Tests
EUR 222

PhosphoWorks™ Fluorimetric Phosphate Assay Kit *Red Fluorescence*
21660 125 Tests
EUR 334

PhosphoWorks™ Fluorimetric Phosphate Assay Kit *Red Fluorescence*
21661 1250 Tests
EUR 2197

PhosphoWorksâ„¢ Colorimetric MESG Phosphate Assay Kit *UV absorption*
21659-200Tests 200 Tests
EUR 334
Description: In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red.

PhosphoWorks™ Colorimetric MESG Phosphate Assay Kit *UV absorption*
21659 200 Tests
EUR 334

Mannose Phosphate Isomerase (MPI) Magnetic Luminex Assay Kit
LKU600969-96T 96T
EUR 966

PicoProbe? Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit
K673-100 each
EUR 927.6

Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit
K670-100 each
EUR 783.6

Amplite® Fluorimetric Glycerol 3-Phosphate (G3P) Assay Kit
13837 200 Tests
EUR 390

Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit
13838 200 Tests
EUR 390

Amplite® Fluorimetric Glycerol 3-Phosphate (G3P) Assay Kit
13837-200Tests 200 Tests
EUR 390
Description: Glycerol 3-Phosphate (G3P) is an important intermediate in the glycolysis metabolic pathway.

Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit
13838-200Tests 200 Tests
EUR 390
Description: Glycerol 3-Phosphate (G3P) is an important intermediate in glycolysis metabolic pathway.

Glucose 6 Phosphate Isomerase (GPI) Magnetic Luminex Assay Kit
LKU605314-96T 96T
EUR 869.4

Glucose 6 Phosphate Isomerase (GPI) Magnetic Luminex Assay Kit
LKU605315-96T 96T
EUR 894.7

Glucose 6 Phosphate Isomerase (GPI) Magnetic Luminex Assay Kit
LKU605316-96T 96T
EUR 944.2

Sphingosine 1 Phosphate Lyase 1 (SGPL1) Magnetic Luminex Assay Kit
LKU601150-96T 96T
EUR 993.6

Sphingosine 1 Phosphate Receptor 1 (S1PR1) Magnetic Luminex Assay Kit
LKU601152-96T 96T
EUR 993.6

Sphingosine 1 Phosphate Receptor 1 (S1PR1) Magnetic Luminex Assay Kit
LKU601154-96T 96T
EUR 966

Sphingosine 1 Phosphate Receptor 2 (S1PR2) Magnetic Luminex Assay Kit
LKU601155-96T 96T
EUR 993.6

Sphingosine 1 Phosphate Receptor 3 (S1PR3) Magnetic Luminex Assay Kit
LKU601160-96T 96T
EUR 993.6

Sphingosine 1 Phosphate Receptor 3 (S1PR3) Magnetic Luminex Assay Kit
LKU601162-96T 96T
EUR 966

Sphingosine 1 Phosphate Receptor 3 (S1PR3) Magnetic Luminex Assay Kit
LKU601165-96T 96T
EUR 1048.8

Sphingosine 1 Phosphate Receptor 4 (S1PR4) Magnetic Luminex Assay Kit
LKU601167-96T 96T
EUR 993.6

Sphingosine 1 Phosphate Receptor 5 (S1PR5) Magnetic Luminex Assay Kit
LKU601170-96T 96T
EUR 993.6

PPDK (Pyruvate, phosphate dikinase) Activity Assay Kit (Fluorometric/Colorimetric)
K456-100 each
EUR 574.8

Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) Magnetic Luminex Assay Kit
LKU606125-96T 96T
EUR 1125.9

Carbamoyl Phosphate Synthase 1, Mitochondrial (CPS1) Magnetic Luminex Assay Kit
LKU603105-96T 96T
EUR 966

Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) Magnetic Luminex Assay Kit
LKU604878-96T 96T
EUR 944.2

Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) Magnetic Luminex Assay Kit
LKU604880-96T 96T
EUR 869.4

Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1 (DPAGT1) Magnetic Luminex Assay Kit
LKU605289-96T 96T
EUR 966

Acid Phosphatase Assay Kit
abx096000-100Assays 100 Assays
EUR 416.4

pNPP Phosphatase Assay Kit
POPN-01K 1000
EUR 408
Description: Quantitative determination of phosphatase activity by colorimetric (405nm) method. Kit size: 1000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

pNPP Phosphatase Assay Kit
POPN-500 500
EUR 249
Description: For quantitative determination of phosphatase enzyme activity. Key Features: High sensitivity and wide linear range. The detection limit is generally 3 ng phosphatase or below. Homogeneous and simple procedure. No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Protein phosphatases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 3 ng.

pNPP Phosphatase Assay Kit
Z5030009 500 assays
EUR 213

pNPP Phosphatase Assay Kit
Z5030010 1,000 assays
EUR 213

Phosphatidylcholine Assay Kit
55R-1443 100 assays
EUR 807.6
Description: Assay Kit for detection of Phosphatidylcholine in the research laboratory

Phosphatidylcholine Assay Kit
STA-600 96 assays
EUR 622.8
Description: Cell Biolabs? Phosphatidylcholine Assay Kit measures the phosphatidylcholine present within serum, plasma, or tissue samples.Samples are compared to a known concentration of phosphatidylcholine standard within the 96-well microtiter plate format.  Samples and standards are incubated for 60 minutes and then read with a standard 96-well fluorometric plate reader.

Alkaline Phosphatase Assay Kit
abx098406-Hitachi7060R190ml2R245ml1 Hitachi 7060; R1: 90ml×2 R2: 45ml×1
EUR 266.4

Alkaline Phosphatase Assay Kit
abx098406-Hitachi7170R132ml4R28ml4 Hitachi 7170; R1: 32ml×4 R2: 8ml×4
EUR 266.4

Alkaline Phosphatase Assay Kit
abx098406-Toshiba120R150ml4R250ml1 Toshiba 120; R1: 50ml×4 R2: 50ml×1
EUR 266.4

Alkaline Phosphatase Assay Kit
abx098406-Toshiba40R150ml4R250ml1 Toshiba 40; R1: 50ml×4 R2: 50ml×1
EUR 266.4

Alkaline Phosphatase Assay Kit
abx096002-100Assays 100 Assays
EUR 379.2

Alkaline Phosphatase Assay Kit
Z5030033 250 assays
EUR 651

Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) Magnetic Luminex Assay Kit
LKU601142-96T 96T
EUR 1048.8

Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) Magnetic Luminex Assay Kit
LKU601144-96T 96T
EUR 966

Sphingosine-1-Phosphate Phosphatase 2 (SGPP2) Magnetic Luminex Assay Kit
LKU601147-96T 96T
EUR 1048.8

Acid Phosphatase Activity Assay Kit
55R-1405 500 assays
EUR 525.6
Description: Assay Kit for detection of Acid Phosphatase in the research laboratory

Acid Phosphatase Activity Assay Kit
55R-1866 500 assays
EUR 580.8
Description: Assay Kit for detection of Acid Phosphatase in the research laboratory

MicroGram Phosphatidylcholine Assay Kit
MPC200K 200 assays
EUR 234.99
Description: This product includes MPC reagent A, MPC reagent B, 100 x MPC reagent C, MPC reagent D, and a standard solution of L-α-phosphatidylcholine (1 mg/ml). The assay kit sufficient for measurement of 200 samples of L-α-phosphatidylcholine using 96-well plates (Costar 3915 and Greiner 655076).

Acid Phosphatase Microplate Assay Kit
DLSM0001 100 Assays
EUR 210
Description: Detection and Quantification of Acid Phosphatase Activity.

Total Phosphatase Microplate Assay Kit
DLSM0176 100 Assays
EUR 140
Description: Detection and Quantification of Total Phosphatase Activity.

Phosphatidylserine Assay Kit (Fluorometric)
K565-100 each
EUR 744

Alkaline Phosphatase Activity Assay Kit
55R-1402 500 assays
EUR 888
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

Alkaline Phosphatase Activity Assay Kit
55R-1406 500 assays
EUR 525.6
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

Phosphatidylglycerol/Cardiolipin Assay Kit
MET-5024 100 assays
EUR 602.4
Description: Our Phosphatidylglycerol/Cardiolipin Assay Kit measures both phosphatidylglycerol and cardiolipin in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidylglycerol and cardiolipin to glycerol, which is then phosphorlyated by glycerol kinase to yield glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit?s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidylglycerol and cardiolipin levels in unknown sampled are determined based on the provided cardiolipin standard curve.

Neutral Phosphatase Microplate Assay Kit
DLSM0207 100 Assays
EUR 210
Description: Detection and Quantification of Acid Phosphatase Activity.

Phosphatidylglycerol Assay Kit (Fluorometric)
K488-100 each
EUR 679.2

Alkaline Phosphatase Microplate Assay Kit
DLSM0003 100 Assays
EUR 175
Description: Detection and Quantification of Alkaline Phosphatase Activity.

QuantiFluo™ Acid Phosphatase Assay Kit
FACP-100 100
EUR 159
Description: For quantitative determination of acid phosphatase activity in biological samples. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.008 to 10 U/L for a 30 minute reaction. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450. Samples: Biological (e.g. serum) samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.008 U/L.

QuantiChrom™ Acid Phosphatase Assay Kit
DACP-100 100
EUR 169
Description: For quantitative determination of acid phosphatase activity in biological samples. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.05 to 60 U/L for a 30 minute reaction. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD405nm. Samples: Biological (e.g. serum) samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.05 U/L.

Phosphatidylethanolamine Assay Kit (Fluorometric)
K499-100 each
EUR 757.2

Acid Phosphatase Cell Cytotoxicity Assay Kit
K937-500 each
EUR 444

QuantiFluo™ Alkaline Phosphatase Assay Kit
QFAP-100 100
EUR 229
Description: For quantitative determination of alkaline phosphatase (ALP) activity using stable 4-methylumbelliferyl phosphate substrate. Key Features: High sensitivity and wide linear range. Use 10 µL sample. Detection limit of 0.02 U/L (20 min reaction). Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: FL360/450nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.02 U/L.

QuantiChrom™ Alkaline Phosphatase Assay Kit
DALP-250 250
EUR 369
Description: For quantitative determination of alkaline phosphatase (ALP) activity using stable p-nitrophenol phosphate substrate. Key Features: High sensitivity and wide linear range. Use 5 µL serum or plasma sample. The detection limit is 2 U/L, linear up to 800 U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 5 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 4 min. Kit size: 250 tests. Detection limit: 2 U/L.

Total Phosphatidic Acid Assay Kit (Fluorometric)
MET-5019 100 assays
EUR 602.4
Description: Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit?s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.

Acid Phosphatase Activity Colorimetric Assay Kit
K411-500 each
EUR 430.8

Acid Phosphatase Activity Fluorometric Assay Kit
K421-500 each
EUR 418.8

Phosphatidylserine (PS) Magnetic Luminex Assay Kit
LKU607510-96T 96T
EUR 1115.5

Phosphatidylinositol (PI) Magnetic Luminex Assay Kit
LKU607445-96T 96T
EUR 1117.8

PicoProbeTM Phosphatidylinositol Assay Kit (Fluorometric)
K750-100 each
EUR 718.8

Phosphatidylcholine Colorimetric/Fluorometric Assay Kit
K576-100 each
EUR 588

Phosphatidylcholine Colorimetric/Fluorometric Assay Kit
K2086-100 100 assays
EUR 602.4

PicoProbeTM Phosphatidic Acid Assay Kit (Fluorometric)
K748-100 each
EUR 744

Alkaline Phosphatase Activity Colorimetric Assay Kit
K2075-500 500 assays
EUR 652.8

Alkaline Phosphatase Activity Fluorometric Assay Kit
K2077-500 500 assays
EUR 435.6

Alkaline Phosphatase Activity Colorimetric Assay Kit
K412-500 each
EUR 646.8

Alkaline Phosphatase Activity Fluorometric Assay Kit
K422-500 each
EUR 418.8

Alkaline Phosphatase (ALP) Magnetic Luminex Assay Kit
LKU600094-96T 96T
EUR 966

Alkaline Phosphatase (ALP) Magnetic Luminex Assay Kit
LKU607078-96T 96T
EUR 917.7

Alkaline Phosphatase (ALP) Magnetic Luminex Assay Kit
LKU607079-96T 96T
EUR 995.9

Alkaline Phosphatase (ALP) Magnetic Luminex Assay Kit
LKU607080-96T 96T
EUR 944.2

Alkaline Phosphatase (ALP) Magnetic Luminex Assay Kit
LKU607081-96T 96T
EUR 995.9

Cellulose, phosphate (Selectacel® phosphate)
19792-100 100g Ask for price
Description: 9015-14-9

Prostatic Acid Phosphatase (PAP) Magnetic Luminex Assay Kit
LKU600999-96T 96T
EUR 869.4

StemTAG Alkaline Phosphatase Activity Assay Kit, Colorimetric
CBA-301 100 assays
EUR 483.6
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Kits provide reagents for quantifying alkaline phosphatase activity in a convenient 96-well plate format, with either colorimetric or fluorescence detection.

StemTAG Alkaline Phosphatase Activity Assay Kit, Fluorometric
CBA-307 100 assays
EUR 540
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Kits provide reagents for quantifying alkaline phosphatase activity in a convenient 96-well plate format, with either colorimetric or fluorescence detection.

Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric)
K829-100 each
EUR 639.6

Sodium Phosphate pH 6.2, 1 M Phosphate (100 ml)
M-CSS-520-6.2 100 ml
EUR 121
Description: Sodium Phosphate pH 6.2, 1 M Phosphate (100 ml)

Sodium Phosphate pH 6.7, 1 M Phosphate (100 ml)
M-CSS-520-6.7 100 ml
EUR 121
Description: Sodium Phosphate pH 6.7, 1 M Phosphate (100 ml)

Sodium Phosphate pH 6.8, 1 M Phosphate (100 ml)
M-CSS-520-6.8 100 ml
EUR 121
Description: Sodium Phosphate pH 6.8, 1 M Phosphate (100 ml)

Sodium Phosphate pH 7.0, 1 M Phosphate (100 ml)
M-CSS-520-7 100 ml
EUR 121
Description: Sodium Phosphate pH 7.0, 1 M Phosphate (100 ml)

Acid Phosphatase 2, Lysosomal (ACP2) Magnetic Luminex Assay Kit
LKU600071-96T 96T
EUR 993.6

Acid Phosphatase 2, Lysosomal (ACP2) Magnetic Luminex Assay Kit
LKU604364-96T 96T
EUR 966

Alkaline Phosphatase, Placental (ALPP) Magnetic Luminex Assay Kit
LKU600650-96T 96T
EUR 917.7

Neutrophil Alkaline Phosphatase (NAP) Magnetic Luminex Assay Kit
LKU603747-96T 96T
EUR 917.7

Phosphatidylinositol Glycan L (PIGL) Magnetic Luminex Assay Kit
LKU607485-96T 96T
EUR 966

Phosphatase And Tensin Homolog (PTEN) Magnetic Luminex Assay Kit
LKU607394-96T 96T
EUR 772.8

Phosphatase And Tensin Homolog (PTEN) Magnetic Luminex Assay Kit
LKU607397-96T 96T
EUR 794.7

Phosphatase And Tensin Homolog (PTEN) Magnetic Luminex Assay Kit
LKU607400-96T 96T
EUR 839.5

Phosphatidylserine Decarboxylase (PISD) Magnetic Luminex Assay Kit
LKU607512-96T 96T
EUR 966

Genorise® Alkaline Phosphatase Activity Colorimetric Assay Kit
GR107015 100 tests
EUR 289

Genorise® Alkaline Phosphatase Activity Fluorometric Assay Kit
GR107016 100 tests
EUR 289

Alkaline Phosphatase, Intestinal (ALPI) Magnetic Luminex Assay Kit
LKU600891-96T 96T
EUR 917.7

Alkaline Phosphatase, Intestinal (ALPI) Magnetic Luminex Assay Kit
LKU600894-96T 96T
EUR 995.9

Density Enhanced Phosphatase 1 (DEP1) Magnetic Luminex Assay Kit
LKU607441-96T 96T
EUR 917.7

Dual Specificity Phosphatase 1 (DUSP1) Magnetic Luminex Assay Kit
LKU601972-96T 96T
EUR 966

Dual Specificity Phosphatase 1 (DUSP1) Magnetic Luminex Assay Kit
LKU601985-96T 96T
EUR 993.6

Dual Specificity Phosphatase 5 (DUSP5) Magnetic Luminex Assay Kit
LKU601993-96T 96T
EUR 993.6

Dual Specificity Phosphatase 5 (DUSP5) Magnetic Luminex Assay Kit
LKU602001-96T 96T
EUR 966

Dual Specificity Phosphatase 6 (DUSP6) Magnetic Luminex Assay Kit
LKU602006-96T 96T
EUR 966

Dual Specificity Phosphatase 9 (DUSP9) Magnetic Luminex Assay Kit
LKU602016-96T 96T
EUR 966

Phosphatidylinositol Trisphosphate (PIP3) Magnetic Luminex Assay Kit
LKU607508-96T 96T
EUR 1269.6

Pyruvate Dehydrogenase Phosphatase (PDP) Magnetic Luminex Assay Kit
LKU606421-96T 96T
EUR 869.4

pH 7 Monobasic K Phosphate/Dibasic Na Phosphate
USP941 1L
EUR 54.05

Phosphatidylinositol 4-phosphate ELISA Kit
DEIA-XYZ11 96T
EUR 1450.8
Description: The Phosphatidylinositol 4-phosphate ELISA Kit is designed to detect and quantify PI(4)P by means of a competitive ELISA format, eliminating the need for radioactivity and thin layer chromatography. The PI(4)P Mass ELISA directly detects PI(4)P over all o

Amplite® Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*
11950 500 Tests
EUR 166

Amplite® Luminometric Alkaline Phosphatase Assay Kit *Luminescence*
11956 100 Tests
EUR 222

Lastly, the rescue experiments confirmed that miR-34b-3p impaired cell viability and migration capability in HUVECs by focusing on PDK1. These findings concluded that miR-34b-3p impaired HUVECs viability and migration in GDM by focusing on PDK1, which could present a novel perspective for the pathogenesis and underlying therapeutic goal for GDM. Valley fever (coccidioidomycosis) is an endemic fungal pneumonia of the North and South American deserts. The causative brokers of Valley fever are the dimorphic fungi Coccidioides immitis and C. posadasii, which develop as mycelia within the atmosphere and as spherules inside the lungs of weak hosts.

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