As one of the widespread malignancy, lung adenocarcinoma (LUAD) is characterised by low 5-year survival price. This analysis aimed to analyze the results of ribonucleotide reductase regulatory subunit M2 (RRM2) on malignant organic behaviors and activation of cGAS/STING pathway. We additionally explored the synergistic sensitization mechanisms of RRM2 and radiotherapy. Bioinformatic instruments have been used to guage the medical significance of RRM2 in LUAD sufferers. The roles of RRM2 in malignant phenotype and DNA injury in LUAD cells have been investigated with cell proliferation, colony formation, immunofluorescence, modified Boyden chamber and comet assays.
The mouse fashions have been used to guage the organic significance of RRM2 in vivo. Cytotoxic T cell infiltration was evaluated through circulation cytometric evaluation and immunohistochemistry staining in C57BL/6 mice. We additionally explored the synergistic results of RRM2 silencing and radiation on LUAD cells with apoptosis assay and immunoblotting in vitro. Bioinformatic evaluation revealed that RRM2 had diagnostic values for LUAD sufferers. Larger ranges of RRM2 predicted worse prognosis. RRM2 silencing inhibited LUAD cell proliferation, invasion and migration.
RRM2 knockdown induced S section arrest and DNA injury. RRM2 silencing induced cyclic GMP-AMP synthase (cGAS)/stimulator of interferon genes (STING) pathway, and the downstream targets have been regulated in a STING-dependent method. Knockdown of RRM2 suppressed tumor progress within the xenograft tumor fashions. RRM2 deficiency elevated CD8 + T cells within the tumor tissues and spleens. Moreover, RRM2 silencing had synergistic results with radiation on inhibiting cell proliferation and selling apoptosis. In the meantime, this mixture promoted the activation of cGAS/STING signaling pathway synergistically, and concurrently elevated expression of IFNβ, CCL5 and CXCL10.
Our outcomes demonstrated that RRM2 silencing had anti-tumor values and activated the cGAS/STING signaling pathway. RRM2 silencing elevated CD8 + T cells infiltration. RRM2 silencing cooperated with radiation to inhibit LUAD cell proliferation, promote apoptosis and improve the activation of cGAS/STING signaling pathway. RRM2 could possibly be a promising goal for tumor regression by most cancers immunotherapy in LUAD.
Identification of the canonical and noncanonical position of miR-143/145 in estrogen-deficient bone loss
Postmenopausal-induced bone loss is especially brought on by declining core transcription elements (TFs) of bone mesenchymal stem cells (BMSCs), however little is thought about how miRNAs regulate chromatin construction reworking of TFs gene to keep up BMSCs perform in bone homeostasis. We examined the serum, salivary and bone samples from Pre- and Submit-menopause ladies by paired evaluation and confirmed canonical ceRNA position of MIR143HG and miR-143/145 complexes in cytoplasm and noncanonical position for SOX2 transcription in nucleus (FISH, qRT-PCR, immunostaining, Luciferase assays and ChIP).
Furthermore, we took benefit of transgenic mice underneath OVX-induced osteoporosis, learning the in vitro and in vivo impact of miR-143/145 deletion on BMSCs perform and bone homeostasis. Final, utilizing miRNA antagonism, antagomiR-143/145 have been delivered into bone marrow to deal with estrogen-deficient bone loss. Right here, we recognized miR-143/145 as potential diagnostic candidates for postmenopausal osteoporosis, and miR-143/145 overexpression impaired BMSCs self-renewing and differentiation perform.
Mechanistically, we confirmed that cytoplasmic miR-143/145 and LncRNA MIR143HG, that managed by ERβ, cooperatively regulated pluripotency genes translation through canonical ceRNA pathway, and MIR143HG cooperates with miR‑143 to nuclear translocation for co-activation of SOX2 transcription through opening promoter chromatin. In the meantime, miR‑143/145 have been shuttled into osteoclasts in extracellular vesicles and triggered osteoclastic exercise by focusing on Cd226 and Srgap2.
Moreover, miR-143/145-/- mice or utilizing chemically‑modified antagomiR-143/145 considerably alleviated estrogen-deficient osteoporosis. Our findings reveal a canonical and noncanonical position of miR-143/145 in controlling BMSCs pluripotency and unfold their twin impact on bone formation and bone resorption, suggesting miR-143/145 as promising therapeutic targets for treating estrogen-deficient bone loss. Present diagnostics for Valley fever are severely missing resulting from poor sensitivity and invasiveness, contributing to a 23-day median time to analysis, and subsequently, new diagnostic instruments are wanted.
MiR-34b-3p Impaired HUVECs Viability and Migration through Concentrating on PDK1 in an In Vitro Mannequin of Gestational Diabetes Mellitus
Gestational diabetes mellitus (GDM) results in poor being pregnant outcomes. The strategies for GDM early analysis and remedy are nonetheless unknown. This research aimed to analyze the expression and diagnostic potential of miR-34b-3p in GDM sufferers and additional analyzed the results of miR-34b-3p on HUVECs viability and migration. The expression of miR-34b-3p was detected in HUVECs of GDM and regular pregnant ladies by qRT-PCR. Then the HUVECs have been remoted from regular pregnant ladies. Excessive glucose (HG) was used to deal with the HUVECs to imitate the GDM in vitro.
The cell viability and migration have been decided by MTT, wound therapeutic assay, and transwell assay. The interplay between miR-34b-3p and PDK1 was evaluated by luciferase exercise assay. Our outcomes confirmed that miR-34b-3p was up-regulated in HUVECs of GDM sufferers. Then the HUVECs have been remoted from regular pregnant ladies they usually have been handled with HG to imitate the GDM in vitro. Curiously, knockdown of miR-34b-3p restored the impairment of HG treatment-induced results in HUVECs. Extra importantly, PDK1 was proved to be a possible goal of miR-34b-3p.
Phosphate Assay Kit (Fluorometric) |
K420-100 |
Biovision |
each |
EUR 418.8 |
Phosphate Assay Kit (Fluorometric) |
STA-686 |
Cell Biolabs |
1000 assays |
EUR 582 |
Description: Our Phosphate Assay Kit (Fluorometric) measures total inorganic phosphate present in lysates, solutions, food, or biological samples in a 96-well fluorescence-based plate reader. 1000 assays/kit. |
PiBlue™ Phosphate Assay Kit |
POPB-500 |
BioAssay Systems |
500 |
EUR 239 |
Description: For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 20 pmoles of phosphate and useful range between 0.4 µM and 50 µM phosphate. Fast and convenient: single reagent "mix-and-measure" assay allows quantitation of free phosphate within 30 minutes. Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well plates. Can be readily automated on HTS liquid handling systems Method: OD620nm (malachite green). Samples: Enzyme reactions with free phosphate releases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 0.4 µM. |
QuantiChrom™ Phosphate Assay Kit |
DIPI-500 |
BioAssay Systems |
500 |
EUR 349 |
Description: For quantitative determination of phosphate and evaluation of drug effects on phosphate metabolism. Key Features: Sensitive and accurate. Linear detection range 0.3 µM (0.0028 mg/dL) to 50 µM (0.47 mg/dL) phosphate in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in cuvet or 96-well plate. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals. Method: OD620nm (malachite green). Samples: Serum, urine, saliva, sweat, tissue culture, food, environment etc. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 3 µg/dL (0.3 µM). |
Malachite Green Phosphate Assay kit |
65-POMG-25H |
Gentaur Genprice |
2500 tests |
EUR 333 |
Malachite Green Phosphate Assay Kit |
abx298878-100Assays |
Abbexa |
100 Assays |
EUR 453.6 |
|
Malachite Green Phosphate Assay Kit |
POMG-25H |
BioAssay Systems |
2500 |
EUR 259 |
Description: For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 1.6 pmoles of phosphate and useful range between 0.02 µM and 40 µM phosphate. Fast and convenient: homogeneous "mix-and-measure" assay allows quantitation of free phosphate within 20 minutes. Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: OD620nm (malachite green). Samples: Biological, environment etc. Species: All. Procedure: Assay takes 30 min. Kit size: 2500 tests. Detection limit: 0.02 µM. |
Malachite Green Phosphate Assay Kit |
Z5030012 |
Biochain |
2,500 assays |
EUR 312 |
Phosphate Fluorometric Assay Kit |
55R-1404 |
Fitzgerald |
100 assays |
EUR 525.6 |
Description: Assay Kit for detection of Phosphate in the research laboratory |
Phosphate Colorimetric Assay Kit |
k2074-500 |
ApexBio |
500 assays |
EUR 351.6 |
Phosphate Colorimetric Assay Kit |
K410-500 |
Biovision |
each |
EUR 379.2 |
Sucrose Phosphate Synthase Assay Kit |
abx298805-100Assays |
Abbexa |
100 Assays |
EUR 754.8 |
|
MESG *Phosphate assay reagent* |
21600 |
AAT Bioquest |
5 mg |
EUR 199 |
MESG *Phosphate assay reagent* |
21600-1MG |
AAT Bioquest |
1 mg |
EUR 77 |
MESG *Phosphate assay reagent* |
21600-1MG-1mg |
AAT Bioquest |
1 mg |
EUR 77 |
|
Description: In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red. |
MESG *Phosphate assay reagent* |
21600-5mg |
AAT Bioquest |
5 mg |
EUR 199 |
|
Description: In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red. |
Phosphoprotein Phosphate Estimation Assay Kit |
BSP061 |
Bio Basic |
2000Assays, 2000preps |
EUR 110.11 |
|
PicoProbe? Phosphate Fluorometric Assay Kit |
K419-100 |
Biovision |
each |
EUR 535.2 |
MicroMolar Phosphate Assay Reagent |
MPA3000 |
ProFoldin |
3000 assays |
EUR 168.93 |
Description: This product includes 450 ml of reagent. It is for 3000 to 5000 assays using 96-well plates or more than 10,000 assays using 384-well plates. It can also be used for measurement of phosphate concentrations using cuvettes and a spectrophotometer. |
PhosphoWorksâ„¢ Colorimetric Phosphate Assay Kit *Blue Color* |
21665-1000Tests |
AAT Bioquest |
1000 Tests |
EUR 222 |
|
Description: Phosphate is involved in many biological reactions. |
Phosphate Assay, Catalog: MA-0105 |
MA-0105 |
Akrivis Bio |
100 wells |
EUR 255 |
PhosphoWorksâ„¢ Fluorimetric Phosphate Assay Kit *Red Fluorescence* |
21660-125Tests |
AAT Bioquest |
125 Tests |
EUR 334 |
|
Description: Cells utilize a wide variety of phosphate (Pi) and polyphosphate esters as enzyme substrates, second messengers, membrane structural components and vital energy reservoirs. |
PhosphoWorksâ„¢ Fluorimetric Phosphate Assay Kit *Red Fluorescence* |
21661-1250Tests |
AAT Bioquest |
1250 Tests |
EUR 2197 |
|
Description: Cells utilize a wide variety of phosphate (Pi) and polyphosphate esters as enzyme substrates, second messengers, membrane structural components and vital energy reservoirs. |
Malachite Green Phosphate Microplate Assay Kit |
DLSM0112 |
DL Develop |
100 Assays |
EUR 262.5 |
Description: Detection and Quantification of Phosphate Content. |
Sucrose Phosphate Synthase Microplate Assay Kit |
DLSM0039 |
DL Develop |
100 Assays |
EUR 595 |
Description: Detection and Quantification of Sucrose Phosphate Synthase Activity. |
PhosphoWorks™ Colorimetric Phosphate Assay Kit *Blue Color* |
21665 |
AAT Bioquest |
1000 Tests |
EUR 222 |
PhosphoWorks™ Fluorimetric Phosphate Assay Kit *Red Fluorescence* |
21660 |
AAT Bioquest |
125 Tests |
EUR 334 |
PhosphoWorks™ Fluorimetric Phosphate Assay Kit *Red Fluorescence* |
21661 |
AAT Bioquest |
1250 Tests |
EUR 2197 |
PhosphoWorksâ„¢ Colorimetric MESG Phosphate Assay Kit *UV absorption* |
21659-200Tests |
AAT Bioquest |
200 Tests |
EUR 334 |
|
Description: In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red. |
PhosphoWorks™ Colorimetric MESG Phosphate Assay Kit *UV absorption* |
21659 |
AAT Bioquest |
200 Tests |
EUR 334 |
Mannose Phosphate Isomerase (MPI) Magnetic Luminex Assay Kit |
LKU600969-96T |
Biomatik Corporation |
96T |
EUR 966 |
PicoProbe? Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit |
K673-100 |
Biovision |
each |
EUR 927.6 |
Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit |
K670-100 |
Biovision |
each |
EUR 783.6 |
Amplite® Fluorimetric Glycerol 3-Phosphate (G3P) Assay Kit |
13837 |
AAT Bioquest |
200 Tests |
EUR 390 |
Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit |
13838 |
AAT Bioquest |
200 Tests |
EUR 390 |
Amplite® Fluorimetric Glycerol 3-Phosphate (G3P) Assay Kit |
13837-200Tests |
AAT Bioquest |
200 Tests |
EUR 390 |
|
Description: Glycerol 3-Phosphate (G3P) is an important intermediate in the glycolysis metabolic pathway. |
Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit |
13838-200Tests |
AAT Bioquest |
200 Tests |
EUR 390 |
|
Description: Glycerol 3-Phosphate (G3P) is an important intermediate in glycolysis metabolic pathway. |
Glucose 6 Phosphate Isomerase (GPI) Magnetic Luminex Assay Kit |
LKU605314-96T |
Biomatik Corporation |
96T |
EUR 869.4 |
Glucose 6 Phosphate Isomerase (GPI) Magnetic Luminex Assay Kit |
LKU605315-96T |
Biomatik Corporation |
96T |
EUR 894.7 |
Glucose 6 Phosphate Isomerase (GPI) Magnetic Luminex Assay Kit |
LKU605316-96T |
Biomatik Corporation |
96T |
EUR 944.2 |
Sphingosine 1 Phosphate Lyase 1 (SGPL1) Magnetic Luminex Assay Kit |
LKU601150-96T |
Biomatik Corporation |
96T |
EUR 993.6 |
Sphingosine 1 Phosphate Receptor 1 (S1PR1) Magnetic Luminex Assay Kit |
LKU601152-96T |
Biomatik Corporation |
96T |
EUR 993.6 |
Sphingosine 1 Phosphate Receptor 1 (S1PR1) Magnetic Luminex Assay Kit |
LKU601154-96T |
Biomatik Corporation |
96T |
EUR 966 |
Sphingosine 1 Phosphate Receptor 2 (S1PR2) Magnetic Luminex Assay Kit |
LKU601155-96T |
Biomatik Corporation |
96T |
EUR 993.6 |
Sphingosine 1 Phosphate Receptor 3 (S1PR3) Magnetic Luminex Assay Kit |
LKU601160-96T |
Biomatik Corporation |
96T |
EUR 993.6 |
Sphingosine 1 Phosphate Receptor 3 (S1PR3) Magnetic Luminex Assay Kit |
LKU601162-96T |
Biomatik Corporation |
96T |
EUR 966 |
Sphingosine 1 Phosphate Receptor 3 (S1PR3) Magnetic Luminex Assay Kit |
LKU601165-96T |
Biomatik Corporation |
96T |
EUR 1048.8 |
Sphingosine 1 Phosphate Receptor 4 (S1PR4) Magnetic Luminex Assay Kit |
LKU601167-96T |
Biomatik Corporation |
96T |
EUR 993.6 |
Sphingosine 1 Phosphate Receptor 5 (S1PR5) Magnetic Luminex Assay Kit |
LKU601170-96T |
Biomatik Corporation |
96T |
EUR 993.6 |
PPDK (Pyruvate, phosphate dikinase) Activity Assay Kit (Fluorometric/Colorimetric) |
K456-100 |
Biovision |
each |
EUR 574.8 |
Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) Magnetic Luminex Assay Kit |
LKU606125-96T |
Biomatik Corporation |
96T |
EUR 1125.9 |
Carbamoyl Phosphate Synthase 1, Mitochondrial (CPS1) Magnetic Luminex Assay Kit |
LKU603105-96T |
Biomatik Corporation |
96T |
EUR 966 |
Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) Magnetic Luminex Assay Kit |
LKU604878-96T |
Biomatik Corporation |
96T |
EUR 944.2 |
Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) Magnetic Luminex Assay Kit |
LKU604880-96T |
Biomatik Corporation |
96T |
EUR 869.4 |
Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1 (DPAGT1) Magnetic Luminex Assay Kit |
LKU605289-96T |
Biomatik Corporation |
96T |
EUR 966 |
Acid Phosphatase Assay Kit |
abx096000-100Assays |
Abbexa |
100 Assays |
EUR 416.4 |
|
pNPP Phosphatase Assay Kit |
POPN-01K |
BioAssay Systems |
1000 |
EUR 408 |
Description: Quantitative determination of phosphatase activity by colorimetric (405nm) method. Kit size: 1000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C. |
pNPP Phosphatase Assay Kit |
POPN-500 |
BioAssay Systems |
500 |
EUR 249 |
Description: For quantitative determination of phosphatase enzyme activity. Key Features: High sensitivity and wide linear range. The detection limit is generally 3 ng phosphatase or below. Homogeneous and simple procedure. No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Protein phosphatases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 3 ng. |
pNPP Phosphatase Assay Kit |
Z5030009 |
Biochain |
500 assays |
EUR 213 |
pNPP Phosphatase Assay Kit |
Z5030010 |
Biochain |
1,000 assays |
EUR 213 |
Phosphatidylcholine Assay Kit |
55R-1443 |
Fitzgerald |
100 assays |
EUR 807.6 |
Description: Assay Kit for detection of Phosphatidylcholine in the research laboratory |
Phosphatidylcholine Assay Kit |
STA-600 |
Cell Biolabs |
96 assays |
EUR 622.8 |
Description: Cell Biolabs? Phosphatidylcholine Assay Kit measures the phosphatidylcholine present within serum, plasma, or tissue samples.Samples are compared to a known concentration of phosphatidylcholine standard within the 96-well microtiter plate format. Samples and standards are incubated for 60 minutes and then read with a standard 96-well fluorometric plate reader. |
Alkaline Phosphatase Assay Kit |
abx098406-Hitachi7060R190ml2R245ml1 |
Abbexa |
Hitachi 7060; R1: 90ml×2 R2: 45ml×1 |
EUR 266.4 |
|
Alkaline Phosphatase Assay Kit |
abx098406-Hitachi7170R132ml4R28ml4 |
Abbexa |
Hitachi 7170; R1: 32ml×4 R2: 8ml×4 |
EUR 266.4 |
|
Alkaline Phosphatase Assay Kit |
abx098406-Toshiba120R150ml4R250ml1 |
Abbexa |
Toshiba 120; R1: 50ml×4 R2: 50ml×1 |
EUR 266.4 |
|
Alkaline Phosphatase Assay Kit |
abx098406-Toshiba40R150ml4R250ml1 |
Abbexa |
Toshiba 40; R1: 50ml×4 R2: 50ml×1 |
EUR 266.4 |
|
Alkaline Phosphatase Assay Kit |
abx096002-100Assays |
Abbexa |
100 Assays |
EUR 379.2 |
|
Alkaline Phosphatase Assay Kit |
Z5030033 |
Biochain |
250 assays |
EUR 651 |
Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) Magnetic Luminex Assay Kit |
LKU601142-96T |
Biomatik Corporation |
96T |
EUR 1048.8 |
Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) Magnetic Luminex Assay Kit |
LKU601144-96T |
Biomatik Corporation |
96T |
EUR 966 |
Sphingosine-1-Phosphate Phosphatase 2 (SGPP2) Magnetic Luminex Assay Kit |
LKU601147-96T |
Biomatik Corporation |
96T |
EUR 1048.8 |
Acid Phosphatase Activity Assay Kit |
55R-1405 |
Fitzgerald |
500 assays |
EUR 525.6 |
Description: Assay Kit for detection of Acid Phosphatase in the research laboratory |
Acid Phosphatase Activity Assay Kit |
55R-1866 |
Fitzgerald |
500 assays |
EUR 580.8 |
Description: Assay Kit for detection of Acid Phosphatase in the research laboratory |
MicroGram Phosphatidylcholine Assay Kit |
MPC200K |
ProFoldin |
200 assays |
EUR 234.99 |
Description: This product includes MPC reagent A, MPC reagent B, 100 x MPC reagent C, MPC reagent D, and a standard solution of L-α-phosphatidylcholine (1 mg/ml). The assay kit sufficient for measurement of 200 samples of L-α-phosphatidylcholine using 96-well plates (Costar 3915 and Greiner 655076). |
Acid Phosphatase Microplate Assay Kit |
DLSM0001 |
DL Develop |
100 Assays |
EUR 210 |
Description: Detection and Quantification of Acid Phosphatase Activity. |
Total Phosphatase Microplate Assay Kit |
DLSM0176 |
DL Develop |
100 Assays |
EUR 140 |
Description: Detection and Quantification of Total Phosphatase Activity. |
Phosphatidylserine Assay Kit (Fluorometric) |
K565-100 |
Biovision |
each |
EUR 744 |
Alkaline Phosphatase Activity Assay Kit |
55R-1402 |
Fitzgerald |
500 assays |
EUR 888 |
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory |
Alkaline Phosphatase Activity Assay Kit |
55R-1406 |
Fitzgerald |
500 assays |
EUR 525.6 |
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory |
Phosphatidylglycerol/Cardiolipin Assay Kit |
MET-5024 |
Cell Biolabs |
100 assays |
EUR 602.4 |
Description: Our Phosphatidylglycerol/Cardiolipin Assay Kit measures both phosphatidylglycerol and cardiolipin in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidylglycerol and cardiolipin to glycerol, which is then phosphorlyated by glycerol kinase to yield glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit?s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidylglycerol and cardiolipin levels in unknown sampled are determined based on the provided cardiolipin standard curve. |
Neutral Phosphatase Microplate Assay Kit |
DLSM0207 |
DL Develop |
100 Assays |
EUR 210 |
Description: Detection and Quantification of Acid Phosphatase Activity. |
Phosphatidylglycerol Assay Kit (Fluorometric) |
K488-100 |
Biovision |
each |
EUR 679.2 |
Alkaline Phosphatase Microplate Assay Kit |
DLSM0003 |
DL Develop |
100 Assays |
EUR 175 |
Description: Detection and Quantification of Alkaline Phosphatase Activity. |
QuantiFluo™ Acid Phosphatase Assay Kit |
FACP-100 |
BioAssay Systems |
100 |
EUR 159 |
Description: For quantitative determination of acid phosphatase activity in biological samples. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.008 to 10 U/L for a 30 minute reaction. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450. Samples: Biological (e.g. serum) samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.008 U/L. |
QuantiChrom™ Acid Phosphatase Assay Kit |
DACP-100 |
BioAssay Systems |
100 |
EUR 169 |
Description: For quantitative determination of acid phosphatase activity in biological samples. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.05 to 60 U/L for a 30 minute reaction. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD405nm. Samples: Biological (e.g. serum) samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.05 U/L. |
Phosphatidylethanolamine Assay Kit (Fluorometric) |
K499-100 |
Biovision |
each |
EUR 757.2 |
Acid Phosphatase Cell Cytotoxicity Assay Kit |
K937-500 |
Biovision |
each |
EUR 444 |
QuantiFluo™ Alkaline Phosphatase Assay Kit |
QFAP-100 |
BioAssay Systems |
100 |
EUR 229 |
Description: For quantitative determination of alkaline phosphatase (ALP) activity using stable 4-methylumbelliferyl phosphate substrate. Key Features: High sensitivity and wide linear range. Use 10 µL sample. Detection limit of 0.02 U/L (20 min reaction). Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: FL360/450nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.02 U/L. |
QuantiChrom™ Alkaline Phosphatase Assay Kit |
DALP-250 |
BioAssay Systems |
250 |
EUR 369 |
Description: For quantitative determination of alkaline phosphatase (ALP) activity using stable p-nitrophenol phosphate substrate. Key Features: High sensitivity and wide linear range. Use 5 µL serum or plasma sample. The detection limit is 2 U/L, linear up to 800 U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 5 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 4 min. Kit size: 250 tests. Detection limit: 2 U/L. |
Total Phosphatidic Acid Assay Kit (Fluorometric) |
MET-5019 |
Cell Biolabs |
100 assays |
EUR 602.4 |
Description: Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit?s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve. |
Acid Phosphatase Activity Colorimetric Assay Kit |
K411-500 |
Biovision |
each |
EUR 430.8 |
Acid Phosphatase Activity Fluorometric Assay Kit |
K421-500 |
Biovision |
each |
EUR 418.8 |
Phosphatidylserine (PS) Magnetic Luminex Assay Kit |
LKU607510-96T |
Biomatik Corporation |
96T |
EUR 1115.5 |
Phosphatidylinositol (PI) Magnetic Luminex Assay Kit |
LKU607445-96T |
Biomatik Corporation |
96T |
EUR 1117.8 |
PicoProbeTM Phosphatidylinositol Assay Kit (Fluorometric) |
K750-100 |
Biovision |
each |
EUR 718.8 |
Phosphatidylcholine Colorimetric/Fluorometric Assay Kit |
K576-100 |
Biovision |
each |
EUR 588 |
Phosphatidylcholine Colorimetric/Fluorometric Assay Kit |
K2086-100 |
ApexBio |
100 assays |
EUR 602.4 |
PicoProbeTM Phosphatidic Acid Assay Kit (Fluorometric) |
K748-100 |
Biovision |
each |
EUR 744 |
Alkaline Phosphatase Activity Colorimetric Assay Kit |
K2075-500 |
ApexBio |
500 assays |
EUR 652.8 |
Alkaline Phosphatase Activity Fluorometric Assay Kit |
K2077-500 |
ApexBio |
500 assays |
EUR 435.6 |
Alkaline Phosphatase Activity Colorimetric Assay Kit |
K412-500 |
Biovision |
each |
EUR 646.8 |
Alkaline Phosphatase Activity Fluorometric Assay Kit |
K422-500 |
Biovision |
each |
EUR 418.8 |
Alkaline Phosphatase (ALP) Magnetic Luminex Assay Kit |
LKU600094-96T |
Biomatik Corporation |
96T |
EUR 966 |
Alkaline Phosphatase (ALP) Magnetic Luminex Assay Kit |
LKU607078-96T |
Biomatik Corporation |
96T |
EUR 917.7 |
Alkaline Phosphatase (ALP) Magnetic Luminex Assay Kit |
LKU607079-96T |
Biomatik Corporation |
96T |
EUR 995.9 |
Alkaline Phosphatase (ALP) Magnetic Luminex Assay Kit |
LKU607080-96T |
Biomatik Corporation |
96T |
EUR 944.2 |
Alkaline Phosphatase (ALP) Magnetic Luminex Assay Kit |
LKU607081-96T |
Biomatik Corporation |
96T |
EUR 995.9 |
Cellulose, phosphate (Selectacel® phosphate) |
19792-100 |
Polysciences Europe GmbH |
100g |
Ask for price |
Description: 9015-14-9 |
Prostatic Acid Phosphatase (PAP) Magnetic Luminex Assay Kit |
LKU600999-96T |
Biomatik Corporation |
96T |
EUR 869.4 |
StemTAG Alkaline Phosphatase Activity Assay Kit, Colorimetric |
CBA-301 |
Cell Biolabs |
100 assays |
EUR 483.6 |
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Kits provide reagents for quantifying alkaline phosphatase activity in a convenient 96-well plate format, with either colorimetric or fluorescence detection. |
StemTAG Alkaline Phosphatase Activity Assay Kit, Fluorometric |
CBA-307 |
Cell Biolabs |
100 assays |
EUR 540 |
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Kits provide reagents for quantifying alkaline phosphatase activity in a convenient 96-well plate format, with either colorimetric or fluorescence detection. |
Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric) |
K829-100 |
Biovision |
each |
EUR 639.6 |
Sodium Phosphate pH 6.2, 1 M Phosphate (100 ml) |
M-CSS-520-6.2 |
MiTeGen |
100 ml |
EUR 121 |
Description: Sodium Phosphate pH 6.2, 1 M Phosphate (100 ml) |
Sodium Phosphate pH 6.7, 1 M Phosphate (100 ml) |
M-CSS-520-6.7 |
MiTeGen |
100 ml |
EUR 121 |
Description: Sodium Phosphate pH 6.7, 1 M Phosphate (100 ml) |
Sodium Phosphate pH 6.8, 1 M Phosphate (100 ml) |
M-CSS-520-6.8 |
MiTeGen |
100 ml |
EUR 121 |
Description: Sodium Phosphate pH 6.8, 1 M Phosphate (100 ml) |
Sodium Phosphate pH 7.0, 1 M Phosphate (100 ml) |
M-CSS-520-7 |
MiTeGen |
100 ml |
EUR 121 |
Description: Sodium Phosphate pH 7.0, 1 M Phosphate (100 ml) |
Acid Phosphatase 2, Lysosomal (ACP2) Magnetic Luminex Assay Kit |
LKU600071-96T |
Biomatik Corporation |
96T |
EUR 993.6 |
Acid Phosphatase 2, Lysosomal (ACP2) Magnetic Luminex Assay Kit |
LKU604364-96T |
Biomatik Corporation |
96T |
EUR 966 |
Alkaline Phosphatase, Placental (ALPP) Magnetic Luminex Assay Kit |
LKU600650-96T |
Biomatik Corporation |
96T |
EUR 917.7 |
Neutrophil Alkaline Phosphatase (NAP) Magnetic Luminex Assay Kit |
LKU603747-96T |
Biomatik Corporation |
96T |
EUR 917.7 |
Phosphatidylinositol Glycan L (PIGL) Magnetic Luminex Assay Kit |
LKU607485-96T |
Biomatik Corporation |
96T |
EUR 966 |
Phosphatase And Tensin Homolog (PTEN) Magnetic Luminex Assay Kit |
LKU607394-96T |
Biomatik Corporation |
96T |
EUR 772.8 |
Phosphatase And Tensin Homolog (PTEN) Magnetic Luminex Assay Kit |
LKU607397-96T |
Biomatik Corporation |
96T |
EUR 794.7 |
Phosphatase And Tensin Homolog (PTEN) Magnetic Luminex Assay Kit |
LKU607400-96T |
Biomatik Corporation |
96T |
EUR 839.5 |
Phosphatidylserine Decarboxylase (PISD) Magnetic Luminex Assay Kit |
LKU607512-96T |
Biomatik Corporation |
96T |
EUR 966 |
Genorise® Alkaline Phosphatase Activity Colorimetric Assay Kit |
GR107015 |
Genorise Scientific |
100 tests |
EUR 289 |
Genorise® Alkaline Phosphatase Activity Fluorometric Assay Kit |
GR107016 |
Genorise Scientific |
100 tests |
EUR 289 |
Alkaline Phosphatase, Intestinal (ALPI) Magnetic Luminex Assay Kit |
LKU600891-96T |
Biomatik Corporation |
96T |
EUR 917.7 |
Alkaline Phosphatase, Intestinal (ALPI) Magnetic Luminex Assay Kit |
LKU600894-96T |
Biomatik Corporation |
96T |
EUR 995.9 |
Density Enhanced Phosphatase 1 (DEP1) Magnetic Luminex Assay Kit |
LKU607441-96T |
Biomatik Corporation |
96T |
EUR 917.7 |
Dual Specificity Phosphatase 1 (DUSP1) Magnetic Luminex Assay Kit |
LKU601972-96T |
Biomatik Corporation |
96T |
EUR 966 |
Dual Specificity Phosphatase 1 (DUSP1) Magnetic Luminex Assay Kit |
LKU601985-96T |
Biomatik Corporation |
96T |
EUR 993.6 |
Dual Specificity Phosphatase 5 (DUSP5) Magnetic Luminex Assay Kit |
LKU601993-96T |
Biomatik Corporation |
96T |
EUR 993.6 |
Dual Specificity Phosphatase 5 (DUSP5) Magnetic Luminex Assay Kit |
LKU602001-96T |
Biomatik Corporation |
96T |
EUR 966 |
Dual Specificity Phosphatase 6 (DUSP6) Magnetic Luminex Assay Kit |
LKU602006-96T |
Biomatik Corporation |
96T |
EUR 966 |
Dual Specificity Phosphatase 9 (DUSP9) Magnetic Luminex Assay Kit |
LKU602016-96T |
Biomatik Corporation |
96T |
EUR 966 |
Phosphatidylinositol Trisphosphate (PIP3) Magnetic Luminex Assay Kit |
LKU607508-96T |
Biomatik Corporation |
96T |
EUR 1269.6 |
Pyruvate Dehydrogenase Phosphatase (PDP) Magnetic Luminex Assay Kit |
LKU606421-96T |
Biomatik Corporation |
96T |
EUR 869.4 |
Phosphatidylinositol 4-phosphate ELISA Kit |
DEIA-XYZ11 |
Creative Diagnostics |
96T |
EUR 1450.8 |
Description: The Phosphatidylinositol 4-phosphate ELISA Kit is designed to detect and quantify PI(4)P by means of a competitive ELISA format, eliminating the need for radioactivity and thin layer chromatography. The PI(4)P Mass ELISA directly detects PI(4)P over all o |
Amplite® Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color* |
11950 |
AAT Bioquest |
500 Tests |
EUR 166 |
Amplite® Luminometric Alkaline Phosphatase Assay Kit *Luminescence* |
11956 |
AAT Bioquest |
100 Tests |
EUR 222 |
Lastly, the rescue experiments confirmed that miR-34b-3p impaired cell viability and migration capability in HUVECs by focusing on PDK1. These findings concluded that miR-34b-3p impaired HUVECs viability and migration in GDM by focusing on PDK1, which could present a novel perspective for the pathogenesis and underlying therapeutic goal for GDM. Valley fever (coccidioidomycosis) is an endemic fungal pneumonia of the North and South American deserts. The causative brokers of Valley fever are the dimorphic fungi Coccidioides immitis and C. posadasii, which develop as mycelia within the atmosphere and as spherules inside the lungs of weak hosts.